ARNT Recombinant Monoclonal Antibody

Name: ARNT Recombinant Monoclonal Antibody
Brand: Beta LifeScience
SKU: BLC-06459A
Availability: InStock

Western Blot | Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate | All lanes: ARNT antibody at 1.83μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 87, 85, 86 KDa | Observed band size: 87 KDa

IHC image of ARNT Recombinant Monoclonal Antibody diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating HIF-1 beta in Hela whole cell lysate | Lane 1: Rabbit control IgG instead of ARNT Recombinant Monoclonal Antibody in Hela whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: ARNT Recombinant Monoclonal Antibody (3μg) + Hela whole cell lysate (500μg) | Lane 3: Hela whole cell lysate (20μg)

Collections: Antibodies

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Product Overview

Product Title	ARNT Recombinant Monoclonal Antibody
Description	The recombinant antibody against ARNT was produced using a synthesized peptide derived from human ARNT as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IP.
Uniprot Id - Alink	P27540
Reactivity	Human
Target Name	ARNT
Target Synonyms	arnT antibody; ARNT protein antibody; ARNT_HUMAN antibody; Aryl hydrocarbon receptor nuclear translocator antibody; bHLHe2 antibody; Class E basic helix-loop-helix protein 2 antibody; Dioxin receptor antibody; Dioxin receptor nuclear translocator antibody; Drnt antibody; HIF 1 beta antibody; HIF 1beta antibody; HIF-1-beta antibody; HIF1-beta antibody; HIF1B antibody; HIF1beta antibody; Hypoxia Inducible Factor 1 antibody; Hypoxia inducible factor 1 beta antibody; Hypoxia-inducible factor 1-beta antibody; nuclear translocator antibody; Tango antibody
Immunogen Description	A synthesized peptide derived from human ARNT
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	Rabbit IgG
Purification Method	Affinity-chromatography purified
Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Liquid
Application	ELISA, WB, IHC, IP
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.