Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein, Active
Beta LifeScience
SKU/CAT #: BLK-02104P-100UG
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein, Active
Beta LifeScience
SKU/CAT #: BLK-02104P-100UG
Collections: Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala18-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1). |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Accession | A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1) |
Target Symbol | GARP&Latent TGF beta 1 Complex |
Synonyms | LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1 |
Species | Cynomolgus |
Expression System | HEK293 |
Tag | C-His-Avi |
Expression Range | Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1) |
Mol. Weight | The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result. |
Form | Lyophilized |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Endotoxin | Less than 1EU per ug by the LAL method. |
Activity | Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. Contact us for detailed testing images. |
Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping | Shipped at ambient temperature. |
Gene Background | GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |