Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein, Active
Beta LifeScience
SKU/CAT #: BLK-00557P-100UG

Biotinylated Human Alkaline Phosphatase (Germ type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein, Active
Beta LifeScience
SKU/CAT #: BLK-00557P-100UG
Collections: High-quality recombinant proteins, Other recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503. |
Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
Accession | P10696 |
Target Symbol | Alkaline Phosphatase (Germ type)/ALPG |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Expression Range | Ile20-Asp503 |
Mol. Weight | The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Tris-Bis PAGE result. |
Form | Lyophilized |
Formulation | Lyophilized from 0.22um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Endotoxin | Less than 1EU per ug by the LAL method. |
Activity | Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 27.8ng/ml determined by ELISA. Contact us for detailed testing images. |
Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Shipping | Shipped at ambient temperature. |
Gene Background | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |