CTLA4 Recombinant Monoclonal Antibody

Name: CTLA4 Recombinant Monoclonal Antibody
Brand: Beta LifeScience
SKU: BLC-06527A
Availability: InStock

Western BlotPositive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, MCF7 whole cell lysateAll lanes: CTLA4 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 25 kDaObserved band size: 25 kDa

Immunofluorescence staining of HEPG2 cell with CTLA4 Recombinant Monoclonal Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay Peak curve showing JK cells surface stained with CTLA4 Recombinant Monoclonal Antibody (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,015 events was performed.

Collections: Antibodies, Co-inhibitory receptors

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Product Overview

Product Title	CTLA4 Recombinant Monoclonal Antibody
Description	The recombinant antibody against CTLA4 was produced using the Recombinant Human CTLA4 protein as the immunogen. This antibody exists as a non-conjugated isotype Mouse IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IF, FC.
Uniprot Id - Alink	P16410
Reactivity	Human
Target Name	CTLA4
Target Synonyms	ALPS5 antibody; CD 152 antibody; CD antibody; CD152 antibody; CD152 antigen antibody; CD152 isoform antibody; Celiac disease 3 antibody; CELIAC3 antibody; CTLA 4 antibody; CTLA-4 antibody; CTLA4 antibody; CTLA4_HUMAN antibody; Cytotoxic T cell associated 4 antibody; Cytotoxic T lymphocyte antigen 4 antibody; Cytotoxic T lymphocyte associated 4 antibody; Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody; Cytotoxic T lymphocyte associated antigen 4 antibody; Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody; Cytotoxic T lymphocyte associated protein 4 antibody; Cytotoxic T lymphocyte associated serine esterase 4 antibody; Cytotoxic T lymphocyte protein 4 antibody; Cytotoxic T-lymphocyte protein 4 antibody; Cytotoxic T-lymphocyte-associated antigen 4 antibody; GRD4 antibody; GSE antibody; ICOS antibody; IDDM12 antibody; insulin-dependent diabetes mellitus 12 antibody; Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody; OTTHUMP00000216623 antibody
Immunogen Description	Recombinant Human CTLA4 protein
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	Mouse IgG
Purification Method	Affinity-chromatography purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IF, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.