Rabbit anti-Human ANXA5 Polyclonal Antibody

Name: Rabbit anti-Human ANXA5 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05966A
Availability: InStock

Western Blot | Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate | All lanes: ANXA5 antibody at 1:3000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 36 kDa | Observed band size: 36 kDa

IHC image of ANXA5 Antibody diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of ANXA5 Antibody diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Collections: Antibodies

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Product Overview

Product Title	Rabbit anti-Human ANXA5 Polyclonal Antibody
Description	The antibody against ANXA5 was raised in rabbit using the Recombinant Human Annexin A5 protein (2-320AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	P08758
Host Species	Rabbit
Reactivity	Human
Target Name	ANXA5
Target Synonyms	Anchorin CII antibody; Annexin 5 antibody; Annexin A5 antibody; Annexin V antibody; Annexin-5 antibody; Annexin5 antibody; AnnexinA5 antibody; AnnexinV antibody; ANX 5 antibody; ANX A5 antibody; ANX5 antibody; ANXA5 antibody; ANXA5_HUMAN antibody; Calphobindin I antibody; CBP-I antibody; Endonexin II antibody; ENX 2 antibody; ENX2 antibody; Lipocortin V antibody; PAP I antibody; PAP-I antibody; Placental anticoagulant protein 4 antibody; Placental anticoagulant protein I antibody; PLACENTAL PROTEIN 4 antibody; PP 4 antibody; Pp4 antibody; RPRGL3 antibody; Thromboplastin inhibitor antibody; VAC-alpha antibody; Vascular anticoagulant-alpha antibody
Immunogen Description	Recombinant Human Annexin A5 protein (2-320AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.