Rabbit anti-Human BUB1B Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-01820A
Immunohistochemistry of paraffin-embedded human endometrial cancer using the antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human endometrial cancer using the antibody at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using BUB1B Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using BUB1B Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating BUB1B in JK whole cell lysate  | Lane 1: Rabbit control IgG (1μg) instead of BUB1B Antibody in Jurkat whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: BUB1B Antibody (6μg) + JK whole cell lysate (500μg) | Lane 3: JK whole cell lysate (10μg)
Immunoprecipitating BUB1B in JK whole cell lysate | Lane 1: Rabbit control IgG (1μg) instead of BUB1B Antibody in Jurkat whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: BUB1B Antibody (6μg) + JK whole cell lysate (500μg) | Lane 3: JK whole cell lysate (10μg)

Rabbit anti-Human BUB1B Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-01820A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Product Title Rabbit anti-Human BUB1B Polyclonal Antibody
Description The antibody against BUB1B was raised in rabbit using the Recombinant Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein (230-394AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF, IP.
Uniprot Id - Alink O60566
Host Species Rabbit
Reactivity Human
Target Name BUB1B
Target Synonyms Beta homolg of S. cerevisiae BUB 1 antibody; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody; BUB 1B antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody; Bub1A antibody; BUB1B antibody; BUB1B_HUMAN antibody; BUB1beta antibody; BUBR1 antibody; Budding Uninhibited by Benzimidazoles 1 beta antibody; Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody; hBUBR1 antibody; MAD3/BUB1 related protein kinase antibody; MAD3/BUB1-related protein kinase antibody; MAD3L antibody; Mitotic checkpoint gene BUB1B antibody; Mitotic checkpoint kinase MAD3L antibody; Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody; MVA1 antibody; OTTHUMP00000160319 antibody; Protein SSK1 antibody; SSK 1 antibody; SSK1 antibody
Immunogen Description Recombinant Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein (230-394AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Application ELISA, IHC, IF, IP
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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