Mouse anti-Human NFE2L2 Monoclonal antibody

Name: Mouse anti-Human NFE2L2 Monoclonal antibody
Brand: Beta LifeScience
SKU: BLC-06402A
Availability: InStock

Western Blot Positive WB detected in: NFE2L2 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: RAW264.7 whole cell lysate Lane 6: K562 whole cell lysateSecondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 68 KDa Observed band size: 68-100 KDa Exposure time: 1min

IHC image of NFE2L2 Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of NFE2L2 Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG labeled by HRP and visualized using 0.05% DAB.

Collections: Antibodies

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Product Overview

Product Title	Mouse anti-Human NFE2L2 Monoclonal antibody
Description	The antibody against NFE2L2 was raised in mouse using the Recombinant Human Nuclear factor erythroid 2-related factor 2 protein (256-605AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG2b, purified by protein A with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF, FC.
Uniprot Id - Alink	Q16236
Host Species	Mouse
Reactivity	Human, Mouse
Target Name	NFE2L2
Target Synonyms	erythroid derived 2 antibody; HEBP1 antibody; like 2 antibody; NF E2 related factor 2 antibody; NF-E2-related factor 2 antibody; NF2L2_HUMAN antibody; NFE2 related factor 2 antibody; NFE2-related factor 2 antibody; Nfe2l2 antibody; Nrf 2 antibody; NRF2 antibody; Nuclear factor (erythroid derived 2) like 2 antibody; Nuclear factor antibody; nuclear factor erythroid 2 like 2 antibody; Nuclear factor erythroid 2 related factor 2 antibody; Nuclear factor erythroid 2-related factor 2 antibody; Nuclear factor erythroid derived 2 like 2 antibody
Immunogen Description	Recombinant Human Nuclear factor erythroid 2-related factor 2 protein (256-605AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	IgG2b
Purification Method	>95%, Protein A purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.