Rabbit anti-Human NLN Polyclonal Antibody

Name: Rabbit anti-Human NLN Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00219A
Availability: InStock

Western Blot | Positive WB detected in: 293 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, LO2 whole cell lysate, A549 whole cell lysate | All lanes: NLN antibody at 3.2µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 81 kDa | Observed band size: 81 kDa

IHC image of NLN Antibody diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with NLN Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Overview

Product Title	Rabbit anti-Human NLN Polyclonal Antibody
Description	The antibody against NLN was raised in rabbit using the Recombinant Human Neurolysin, mitochondrial protein (109-241AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	Q9BYT8
Host Species	Rabbit
Reactivity	Human
Target Name	NLN
Target Synonyms	AGTBP antibody; Angiotensin binding protein antibody; Angiotensin-binding protein antibody; DKFZp564F123 antibody; EC 3.4.24.16 antibody; EP24.16 antibody; FLJ23002 antibody; KIAA1226 antibody; MEP antibody; Microsomal endopeptidase antibody; mitochondrial antibody; Mitochondrial oligopeptidase M antibody; MOP antibody; NEUL_HUMAN antibody; neurolysin (metallopeptidase M3 family) antibody; Neurolysin antibody; Neurolysin, mitochondrial precursor antibody; Neurotensin endopeptidase antibody; Nln antibody
Immunogen Description	Recombinant Human Neurolysin, mitochondrial protein (109-241AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.