Mouse anti-Human NOS2 Monoclonal antibody

Name: Mouse anti-Human NOS2 Monoclonal antibody
Brand: Beta LifeScience
SKU: BLC-06394A
Availability: InStock

Western Blot Positive WB detected in: Hela whole cell lysate All lanes: NOS2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 131, 127 kDa Observed band size: 131 KDa Exposure time：5min

Overlay Peak curve showing Hela cells stained with NOS2 Monoclonal Antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Overlay Peak curve showing HepG2 cells stained with NOS2 Monoclonal Antibody (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Product Overview

Product Title	Mouse anti-Human NOS2 Monoclonal antibody
Description	The antibody against NOS2 was raised in mouse using the Recombinant Human Nitric oxide synthase, inducible protein (1-200AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG2b, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, FC.
Uniprot Id - Alink	P35228
Host Species	Mouse
Reactivity	Human
Target Name	NOS2
Target Synonyms	HEP-NOS antibody; Hepatocyte NOS antibody; HEPNOS antibody; inducible antibody; Inducible nitric oxide synthase antibody; Inducible NO synthase antibody; Inducible NOS antibody; iNOS antibody; MAC NOS antibody; Macrophage NOS antibody; Nitric oxide synthase 2 inducible antibody; Nitric oxide synthase 2 inducible macrophage antibody; nitric oxide synthase 2A (inducible, hepatocytes) antibody; Nitric oxide synthase antibody; Nitric oxide synthase inducible antibody; nitric oxide synthase, macrophage antibody; NOS 2 antibody; NOS antibody; Nos II antibody; NOS type II antibody; nos2 antibody; NOS2_HUMAN antibody; NOS2A antibody; NOS2A, Inducible, Hepatocyte antibody; Peptidyl-cysteine S-nitrosylase NOS2 antibody
Immunogen Description	Recombinant Human Nitric oxide synthase, inducible protein (1-200AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	IgG2b
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.