Mouse anti-Human OCT4 Monoclonal Antibody

Name: Mouse anti-Human OCT4 Monoclonal Antibody
Brand: Beta LifeScience
SKU: BLC-06426A
Availability: InStock

IHC image of OCT4 Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Collections: Antibodies

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Product Overview

Product Title	Mouse anti-Human OCT4 Monoclonal Antibody
Description	The antibody against OCT4 was raised in mouse using the Recombinant Human POU domain, class 5, transcription factor 1 protein (1-360AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG2b, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF, FC.
Uniprot Id - Alink	Q01860
Host Species	Mouse
Reactivity	Human, Mouse, Rat
Target Name	OCT4
Target Synonyms	Octamer binding transcription factor 4 antibody; MGC22487 antibody; Oct 3 antibody; Oct 4 antibody; Oct-3 antibody; Oct-4 antibody; OCT3 antibody; Oct4 antibody; Octamer binding protein 3 antibody; Octamer binding protein 4 antibody; Octamer binding transcription factor 3 antibody; Octamer-binding protein 3 antibody; Octamer-binding protein 4 antibody; Octamer-binding transcription factor 3 antibody; OTF 3 antibody; OTF 4 antibody; OTF-3 antibody; OTF3 antibody; OTF4 antibody; PO5F1_HUMAN antibody; POU class 5 homeobox 1 antibody; POU domain class 5 transcription factor 1 antibody; POU domain transcription factor OCT4 antibody; POU domain, class 5, transcription factor 1 antibody; POU-type homeodomain-containing DNA-binding protein antibody; POU5F1 antibody
Immunogen Description	Recombinant Human POU domain, class 5, transcription factor 1 protein (1-360AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Monoclonal
Isotype	IgG2b
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF, FC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.