Rabbit anti-Human PER2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-05185A
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Period Circadian Protein 2 (Phospho-Ser662) antibody. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using Period Circadian Protein 2 (Phospho-Ser662) antibody. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 3T3lbnotHeLa and K562 cells, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis of extracts from 3T3lbnotHeLa and K562 cells, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Period Circadian Protein 2 (Phospho-Ser662) antibody. The picture on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Period Circadian Protein 2 (Phospho-Ser662) antibody. The picture on the right is treated with the synthesized peptide.

Rabbit anti-Human PER2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-05185A

Collections: Antibodies

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Product Overview

Product Title Rabbit anti-Human PER2 Polyclonal Antibody
Description The antibody against PER2 was raised in rabbit using the Peptide sequence around phosphorylation site of serine 662 (A-E-S(p)-V-A) derived from Human Period Circadian Protein 2. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink O15055
Host Species Rabbit
Reactivity Human, Mouse
Target Name PER2
Target Synonyms Circadian clock protein PERIOD 2 antibody; FASPS antibody; FASPS1 antibody; hPER 2 antibody; hPER2 antibody; KIAA0347 antibody; OTTHUMP00000164476 antibody; PER 2 antibody; PER2 antibody; PER2_HUMAN antibody; Period 2 antibody; Period 2 isoform 1 antibody; Period circadian clock 2 antibody; Period circadian protein 2 antibody; Period circadian protein homolog 2 antibody; Period homolog 2 (Drosophila) antibody; Period homolog 2 antibody; Period; Drosophila; homolog of; 2 antibody; Period2 antibody
Immunogen Description Peptide sequence around phosphorylation site of serine 662 (A-E-S(p)-V-A) derived from Human Period Circadian Protein 2.
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Application ELISA, WB, IHC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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