Rabbit anti-Human PIK3R2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-01115A
Western Blot | Positive WB detected in: Jurkat whole cell lysate | All lanes: PIK3R2 antibody at 4μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 82 kDa | Observed band size: 82 kDa
Western Blot | Positive WB detected in: Jurkat whole cell lysate | All lanes: PIK3R2 antibody at 4μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 82 kDa | Observed band size: 82 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using PIK3R2 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using PIK3R2 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using PIK3R2 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using PIK3R2 Antibody at dilution of 1:100

Rabbit anti-Human PIK3R2 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-01115A

Collections: Antibodies

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Product Overview

Product Title Rabbit anti-Human PIK3R2 Polyclonal Antibody
Description The antibody against PIK3R2 was raised in rabbit using the Recombinant Human Phosphatidylinositol 3-kinase regulatory subunit beta protein (75-125AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink O00459
Host Species Rabbit
Reactivity Human
Target Name PIK3R2
Target Synonyms p85 antibody; p85 beta antibody; P85B antibody; P85B_HUMAN antibody; Phosphatidylinositol 3 kinase antibody; Phosphatidylinositol 3 kinase regulatory beta subunit antibody; Phosphatidylinositol 3 kinase regulatory subunit beta antibody; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody; Phosphatidylinositol 3 kinase; regulatory subunit; polypeptide 2 (p85 beta) antibody; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody; Phosphatidylinositol 3-kinase regulatory subunit beta antibody; Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody; Phosphoinositide 3 kinase regulatory subunit 2 antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody; Phosphoinositide 3 kinase; regulatory subunit 2 (beta) antibody; Phosphoinositide 3 kinase; regulatory subunit 2 (p85 beta) antibody; PI3 kinase p85 beta subunit antibody; PI3 kinase p85 subunit beta antibody; PI3-kinase regulatory subunit beta antibody; PI3-kinase subunit p85-beta antibody; PI3K antibody; PI3K regulatory subunit beta antibody; PIK3R 2 antibody; PIK3R2 antibody; PtdIns 3 kinase p85 beta antibody; PtdIns-3-kinase regulatory subunit beta antibody; PtdIns-3-kinase regulatory subunit p85-beta antibody
Immunogen Description Recombinant Human Phosphatidylinositol 3-kinase regulatory subunit beta protein (75-125AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Application ELISA, WB, IHC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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