Rabbit anti-Human PMEL Polyclonal Antibody

Name: Rabbit anti-Human PMEL Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-05960A
Availability: InStock

IHC image of PMEL Antibody diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PMEL Antibody diluted at 1:200 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Product Title	Rabbit anti-Human PMEL Polyclonal Antibody
Description	The antibody against PMEL was raised in rabbit using the Recombinant Human Melanocyte protein PMEL protein (25-467AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink	P40967
Host Species	Rabbit
Reactivity	Human
Target Name	PMEL
Target Synonyms	95 kDa melanocyte specific secreted glycoprotein antibody; 95 kDa melanocyte-specific secreted glycoprotein antibody; D12S53E antibody; gp100 antibody; M-beta antibody; ME20 antibody; ME20 M/ME20 S antibody; ME20-M antibody; ME20-S antibody; ME20M antibody; ME20M/ME20S antibody; ME20S antibody; Melanocyte lineage specific antigen GP100 antibody; Melanocyte protein mel 17 antibody; Melanocyte protein Pmel 17 antibody; Melanocyte protein Pmel 17 precursor antibody; Melanocytes lineage-specific antigen GP100 antibody; Melanoma associated ME20 antigen antibody; Melanoma gp100 antibody; Melanoma-associated ME20 antigen antibody; Melanosomal matrix protein 17 antibody; Melanosomal matrix protein17 antibody; P1 antibody; p100 antibody; p26 antibody; PMEL 17 antibody; PMEL antibody; PMEL_HUMAN antibody; PMEL17 antibody; Premelanosome protein antibody; Secreted melanoma-associated ME20 antigen antibody; SI antibody; SIL antibody; SILV antibody; Silver (mouse homolog) like antibody; Silver homolog antibody; Silver locus protein homolog antibody; Silver, mouse, homolog of antibody
Immunogen Description	Recombinant Human Melanocyte protein PMEL protein (25-467AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form	Liquid
Application	ELISA, WB, IHC
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.