Rabbit anti-Human RHOU Polyclonal Antibody

Name: Rabbit anti-Human RHOU Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00524A
Availability: InStock

IHC image of the antibody diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of RHOU Antibody diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with RHOU Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Overview

Product Title	Rabbit anti-Human RHOU Polyclonal Antibody
Description	The antibody against RHOU was raised in rabbit using the Recombinant Human Rho-related GTP-binding protein RhoU protein (1-48AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF.
Uniprot Id - Alink	Q7L0Q8
Host Species	Rabbit
Reactivity	Human
Target Name	RHOU
Target Synonyms	ARHU antibody; CDC42 L1 antibody; CDC42 like GTPase antibody; CDC42-like GTPase 1 antibody; CDC42L1 antibody; G28K antibody; GTP binding protein like 1 antibody; GTP binding protein SB128 antibody; GTP-binding protein-like 1 antibody; hG28K antibody; mG28K antibody; Ras homolog gene family member U antibody; Ras homolog gene family member U isoform CRA a antibody; Ras like gene family member U antibody; Rho GTPase like protein ARHU antibody; Rho GTPase-like protein ARHU antibody; Rho related GTP binding protein RhoU antibody; Rho-related GTP-binding protein RhoU antibody; Rhou antibody; RHOU_HUMAN antibody; Ryu GTPase antibody; Wnt-1 responsive Cdc42 homolog 1 antibody; Wnt1 responsive Cdc42 homolog antibody; WRCH 1 antibody; WRCH-1 antibody
Immunogen Description	Recombinant Human Rho-related GTP-binding protein RhoU protein (1-48AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	>95%, Protein G purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.