Recombinant Antigen Mpt64 (M. Tuberculosis/H37Rv)

Name: Recombinant Antigen Mpt64 (M. Tuberculosis/H37Rv)
Brand: Beta LifeScience
SKU: BLIT-1005
Availability: InStock

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

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Product Overview

Tag	N/A
Host Species	M. Tuberculosis
Description	Antigen Mpt64 (M. Tuberculosis/H37Rv) was expressed in E.coli; C-terminal 6xHis fusion protein of antigen Mpt64(Rv1980c) (M. Tuberculosis/H37Rv)(a.a.1-232) (Genbank Accession No. NP_216496). Predicted possible vaccine candidate, which is immunogenic protein. Exported protein containing an N-terminal signal sequence.
Source	E.coli
Purity	>95% as determined by SDS PAGE
Formulation	Each vial contains 100 µg of lyophilized protein in PBS with 8M Urea. Reconstitute the protein with 100 µl of Millipore water.
Applications	Antibody ELISA; immunogen; WB, etc.
Usage	For Research Use Only

Target Details

Subcellular Location	Secreted.
Database References	KEGG: mtu:Rv1980c STRING: 83332.Rv1980c

Gene Functions References

Aptamers against Mtb secreted protein MPT64 were successfully selected and could be used as an alternative for detection and diagnosis of tuberculosis. PMID: 28454652
we found that polymorphisms of the mpt64 gene in the MTBC may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion. PMID: 23390287
CFP21 (cutinase precursor) and MPT64 fusion protein stimulate higher level of interferon (IFN)-gamma in tuberculin skin test (TST)-positive healthy population than in TST-negative healthy population. PMID: 21340709
Data show that Recombinant Mycobacterium vaccae secreted MPT64 could induce high level humoral and cell mediated immune responses in mice and could be used as a candidate of new vaccine against TB. PMID: 19257983
Recombinant rMPT64 elicits a specific immune response indistinguishable from that of MPB64 purified from BCG Tokyo culture filtrates. PMID: 16216527

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.