Recombinant Antigen Rv0477 (M. Tuberculosis/H37Rv)

Name: Recombinant Antigen Rv0477 (M. Tuberculosis/H37Rv)
Brand: Beta LifeScience
SKU: BLIT-1000
Availability: InStock

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	N/A
Host Species	M. Tuberculosis
Description	Antigen Rv0477 (M. Tuberculosis/H37Rv) was expressed in E.coli; N-terminal GST fusion protein with C-terminal 6xHis tagged antigen Rv0477 (M. Tuberculosis/H37Rv)(a.a.1-152) (Genbank Accession No. NP_214991). Possible conserved secreted protein. Contains possible N-terminal signal sequence. A core mycobacterial gene conserved in mycobacterial strains.
Source	E.coli
Purity	>95% as determined by SDS PAGE
Formulation	Each vial contains 100 µg of lyophilized protein in PBS with 8M Urea. Reconstitute the protein with 100 µl of Millipore water.
Applications	Antibody ELISA; immunogen; WB, etc.
Usage	For Research Use Only

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.