Recombinant Arabidopsis Thaliana Catalase-2 (CAT2) Protein (His-SUMO)

Name: Recombinant Arabidopsis Thaliana Catalase-2 (CAT2) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03876P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Recombinant Arabidopsis Thaliana Catalase-2 (CAT2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P25819
Target Symbol	CAT2
Synonyms	CAT2; CAT; At4g35090; M4E13.140Catalase-2; EC 1.11.1.6
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MDPYKYRPASSYNSPFFTTNSGAPVWNNNSSMTVGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLNMFTFLFDDIGIPQDYRHMDGSGVNTYMLINKAGKAHYVKFHWKPTCGVKSLLEEDAIRVGGTNHSHATQDLYDSIAAGNYPEWKLFIQIIDPADEDKFDFDPLDVTKTWPEDILPLQPVGRMVLNKNIDNFFAENEQLAFCPAIIVPGIHYSDDKLLQTRVFSYADTQRHRLGPNYLQLPVNAPKCAHHNNHHEGFMNFMHRDEEVNYFPSRYDQVRHAEKYPTPPAVCSGKRERCIIEKENNFKEPGERYRTFTPERQERFIQRWIDALSDPRITHEIRSIWISYWSQADKSLGQKLASRLNVRPSI
Expression Range	1-492aa
Protein Length	Full Length
Mol. Weight	72.9kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Subcellular Location	Cytoplasm. Peroxisome. Glyoxysome.
Protein Families	Catalase family
Database References	KEGG: ath:AT4G35090 STRING: 3702.AT4G35090.1 UniGene: At.24350

Gene Functions References

The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild-type and the cat2/3 double mutants. PMID: 29575603
CAT2 functions in early seedling growth by scavenging hydrogen peroxide and stimulating ACX2/3 activity. PMID: 28722222
SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions PMID: 27432873
CAT2 role in photorespiration. PMID: 27225899
CAT2 coordinates salicylic acid repression of auxin accumulation and jasmonic acid biosynthesis in plant defense PMID: 28182949
The CAT2 promoter is regulated by the ACGT motif (Box2) rather than by the G-Box binding motif (Box1) in the vegetative phase of development. PMID: 27734290
CAT2 and NCA1 promote autophagy-dependent cell death in Arabidopsis. PMID: 24285797
The hyponastic leaves of cat2-1 reveal crosstalk between H2 O2 and auxin signalling that is mediated by changes in glutathione redox status. PMID: 23738953
NADP-ME2 transcripts accumulated in response to ozone or in mutants undergoing intracellular oxidative stress. Loss of NADP-ME2 function had little effect on cat2-triggered changes in glutathione or NADPH. PMID: 23851193
Introducing the cad2 mutation into cat2 shows that the cad2 mutation overrode the main features of the cat2 effect on thiol profiles. PMID: 23210597
Data indicate that in three different configurations, primary silencing signals were able to direct in trans methylation of chimeric transgenes and the CATALASE2 (CAT2) endogene. PMID: 23480471
the roles of catalase (CAT) in abscisic acid (ABA)-induced stomatal closure PMID: 21979081
Studies indicate that in apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. PMID: 21220338
Using CAT2 as an example, it was demonstrated that increasing the distance between direct and adjacent target sequences in a transgenic primary target delays the onset of silencing of a secondary target gene PMID: 16891552
CAT2 expression is down-regulated during leaf senescence PMID: 17080932
Data suggest that the interaction of SOS2 with both NDPK2 and CATs2 and 3 reveals a point of cross talk between salt stress response and other signaling factors including H2O2. PMID: 17785451
Data show that cat2-1 mutant phenotype include more H(2)O(2), reduced size, paler green color, smaller secondary roots and increased sensitivity to H(2)O(2), NaCl, norspermidine, high light and cold stress. The germination is more tolerant to lithium. PMID: 17931347

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.