Recombinant Arabidopsis Thaliana Dehydration-Responsive Element-Binding Protein 2C (DREB2C) Protein (His)

Name: Recombinant Arabidopsis Thaliana Dehydration-Responsive Element-Binding Protein 2C (DREB2C) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04868P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Arabidopsis Thaliana Dehydration-Responsive Element-Binding Protein 2C (DREB2C) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8LFR2
Target Symbol	DREB2C
Synonyms	DREB2C; ERF048; At2g40340; T07M07.24; T3G21.11Dehydration-responsive element-binding protein 2C; Protein DREB2C
Species	Arabidopsis thaliana(Mouse-ear cress)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	MPSEIVDRKRKSRGTRDVAEILRQWREYNEQIEAESCIDGGGPKSIRKPPPKGSRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAYDEAAKAIYGQSARLNLPEITNRSSSTAATATVSGSVTAFSDESEVCAREDTNASSGFGQVKLEDCSDEYVLLDSSQCIKEELKGKEEVREEHNLAVGFGIGQDSKRETLDAWLMGNGNEQEPLEFGVDETFDINELLGILNDNNVSGQETMQYQVDRHPNFSYQTQFPNSNLLGSLNPMEIAQPGVDYGCPYVQPSDMENYGIDLDHRRFNDLDIQDLDFGGDKDVHGST
Expression Range	1-341aa
Protein Length	Full Length
Mol. Weight	43.9 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and abscisic acid-inducible transcription.
Subcellular Location	Nucleus.
Protein Families	AP2/ERF transcription factor family, ERF subfamily
Database References	KEGG: ath:AT2G40340 STRING: 3702.AT2G40340.1 UniGene: At.37068

Gene Functions References

These results demonstrate that VOZ1 functions as a negative regulator of heat stress (HS)-inducible DREB2C signaling by blocking access to the AP2 DNA-binding domain of DREB2C. PMID: 29536220
DREB2C functions as transcriptional activator that promotes NaCl tolerance, in part through upregulation of the stress-responsive gene COR15A. PMID: 24737413
results suggest that DREB2C is a stress- and ABA-inducible gene that acts as a positive regulator of ABA biosynthesis in germinating seeds through activating NCED9 expression PMID: 25150152
We propose that AtCYS4 functions in thermotolerance under the control of the DREB2C cascade PMID: 23868510
The TATA-proximal region and some latent trans-acting factors may cooperate in heat shock-induced activation of the Arabidopsis DREB2C promoter. PMID: 22716647
These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis. PMID: 22286229
these results indicate that DREB2C and HsfA3 are key players in regulating the heat tolerance of Arabidopsis. PMID: 20849812
Overexpression of DREB2C affects the expression of stress-responsive genes and affects ABA sensitivity. PMID: 20395451
identify possible targets of DREB2C transcription factors at the protein level PMID: 19830397
Data suggest that DREB2C is a regulator of heat stress tolerance in Arabidopsis. PMID: 17716623

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.