Recombinant Arabidopsis Thaliana Udp-Glycosyltransferase 89C1 (UGT89C1) Protein (His)

Name: Recombinant Arabidopsis Thaliana Udp-Glycosyltransferase 89C1 (UGT89C1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-02426P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Arabidopsis Thaliana Udp-Glycosyltransferase 89C1 (UGT89C1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q9LNE6
Target Symbol	UGT89C1
Synonyms	UGT89C1; At1g06000; T21E18.5; Flavonol 7-O-rhamnosyltransferase; EC 2.4.1.-; UDP-glycosyltransferase 89C1; UDP-rhamnose: flavonol 7-O-rhamnosyltransferase
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	MTTTTTKKPHVLVIPFPQSGHMVPHLDLTHQILLRGATVTVLVTPKNSSYLDALRSLHSPEHFKTLILPFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLSPWINKVADAFSIKSISFLPINAHSISVMWAQEDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWTVGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAALEKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAVGSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDKLARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELVAEMCL
Expression Range	1-435aa
Protein Length	Full Length
Mol. Weight	51.6 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Flavonol 7-O-rhamnosyltransferase that catalyzes the transfer of rhamnose from UDP-rhamnose to the 7-OH position of 3-O-glycosylated flavonols, such as kaempferol 3-O-rhamnoside, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-rhamnoside and isorhamnetin 3-O-glucoside. Is able to glycosylate the flavonols quercetin and kaempferol to yield quercetin 7-O-rhamnoside and kaempferol 7-O-rhamnoside. Shows a strict specificity for UDP-rhamnose as sugar donor. Does not act on 3-O-glycosylated anthocyanins. The accumulation of kaempferol 3-O-rhamnoside-7-O-rhamnoside inhibits basipetal auxin transport, which influences auxin distribution and plant organ development.
Protein Families	UDP-glycosyltransferase family
Database References	KEGG: ath:AT1G06000 STRING: 3702.AT1G06000.1 UniGene: At.28159
Tissue Specificity	Highly expressed in floral buds. Expressed in stems, leaves and flowers. Expressed at low levels in roots and siliques. Expressed on the adaxial side of cotyledons and emerging leaves, in trichomes, root columella cells, and the late elongation/early diff

Gene Functions References

Several rol1-2 suppressor mutants of the 7-O-rhamnosyltransferase locus UGT89C1 were identified. Levels of auxin conjugates and catabolites are strongly increased in the ugt89c1 mutant background. PMID: 26742840

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.