Recombinant CRISPR-Cas12a protein

Name: Recombinant CRISPR-Cas12a protein
Brand: Beta LifeScience
SKU: BLC-0298P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Host Species	ACISB
Accession	CRISPR-Cas12a
Synonym	CRISPR-associated endonuclease Cas12a, AsCpf1, CRISPR-associated endonuclease Cpf1, cas12a, cpf1, cas12a
Background	CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cleave DNA, as components of bacterial immune systems. Cas12 is a compact and efficient enzyme that creates staggered cuts in dsDNA. Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type V-A CRISPR-Cas anti-phage immune system that can be repurposed for genome editing. Cas12a can bind and cut dsDNA targets with high specificity in vivo, making it an ideal candidate for expanding the arsenal of enzymes used in precise genome editing.
Description	Recombinant CRISPR-Cas12a protein is expressed in E.coli and it can be used for genome engineering. Cas12a is a single crRNA-guided endonuclease which can recognize a 5-€² T-rich protospacer adjacent motif (PAM).
Source	E.coli
Purity	>95% by SDS-PAGE and SEC-HPLC
Formulation	Liquid Solution
Size	100pmol(20ul)/2000pmol(250ul)
Usage	For Research Use Only
Storage	Store, frozen at -20~-80°C for longer periods of time. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.