Recombinant Fruit Fly Accessory Gland-Specific Peptide 70A (SP)

Name: Recombinant Fruit Fly Accessory Gland-Specific Peptide 70A (SP)
Brand: Beta LifeScience
SKU: BLC-10546P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

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Product Overview

Description	Recombinant Fruit Fly Accessory Gland-Specific Peptide 70A (SP) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P05623
Target Symbol	SP
Synonyms	SP; Acp70A; PAPB; CG17673Accessory gland-specific peptide 70A; Paragonial peptide B; Sex peptide; SP
Species	Drosophila melanogaster (Fruit fly)
Expression System	Yeast
Tag	Tag-Free
Target Protein Sequence	WEWPWNRKPTKFPIPSPNPRDKWCRLNLGPAWGGRC
Expression Range	20-55aa
Protein Length	Full Length of Mature Protein
Mol. Weight	4.3kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Male seminal protein which triggers short- and long-term post-mating behavioral responses (PMR) in female Drosophila. Binds initially to sperm where it is later cleaved to release an active peptide within the female reproductive tract. Signals via the sex peptide receptor (SPR) in female flies; may also act via other receptors. Moderates the activity of distinct neuronal circuitries in the female genital tract to promote specific PMRs including: enhanced ovulation, increased egg laying rate, increased feeding/foraging rate, induced antimicrobial peptide synthesis, reduced mating receptivity, reduced day-time sleep and reduced lifespan in multiple mated females.
Subcellular Location	Secreted.
Protein Families	Drosophila sex peptide family
Database References	KEGG: dme:Dmel_CG17673 STRING: 7227.FBpp0075572 UniGene: Dm.2898
Tissue Specificity	Main cells of the accessory glands of males (paragonial gland).

Gene Functions References

Molecular Functions and Cross-Species Activity of Sex-Peptide in Drosophila PMID: 26022240
sex peptide is required for normal magnitude and persistence of postmating responses PMID: 12893873
sex peptide is the major agent eliciting the short-term and the long-term postmating responses PMID: 12897240
Drosophila sex-peptide stimulates female innate immune system after mating via the Toll and Imd pathways. PMID: 16169493
Copulation strongly stimulates female food intake and Sex Peptide is the main agent controlling this behavioral paradigm. PMID: 16581515
SP acts by chemical mimicry of sugar components of the bacterial cell wall. Thus, SP may induce the immune system via pattern recognition receptors. PMID: 17922838
Data show significant variation in expression levels of the SP gene Acp70A across D. melanogaster isolines. PMID: 19640319

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.