Recombinant H7N9 Anhui Recombinant

Beta LifeScience SKU/CAT #: BLIT-0747

Recombinant H7N9 Anhui Recombinant

Beta LifeScience SKU/CAT #: BLIT-0747
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag N/A
Host Species H7N9
Background H7N9 is a strain of avian influenza virus or bird flu virus which is a species of Influenza virus A. Avian influenza A H7 viruses generally circulate amongst avian populations with some variants known to sporadically infect humans. An H7N9 virus was initially reported to have infected humans in March 2013, in China. It is known that influenza tends to occur during the winter months, and the 2nd wave, which began in October, was fueled by a surge in poultry production planned for Chinese New Year feasts which began at the end of January. WHO has identified H7N9 as "...an unusually dangerous virus for humans." The majority of the cases resulted in severe respiratory illness, with a mortality rate of approximately 30%. It has been determined that many of the human cases of H7N9 seem to have a link to live bird markets. Since H7N9 virus does not kill poultry, it makes observation much more difficult.
Description Recombinant Full-Length H7N9 Anhui 01/2013 is glycosylated with N-linked sugars, produced using baculovirus vectors in insect cells.
Source Insect cells
Purity Greater than 90.0% as determined by SDS-PAGE.
Formulation The Recombinant H7N9 A/ Anhui 01/2013 solution contains 10mM Sodium phosphate, pH 7.1,150mM NaCl and 0.005% Tween-20.
Applications Antibody ELISA; immunogen; WB, etc.
Usage For Research Use Only

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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