Recombinant Helicobacter Pylori Cytotoxicity-Associated Immunodominant Antigen (CAGA) Protein (His)

Name: Recombinant Helicobacter Pylori Cytotoxicity-Associated Immunodominant Antigen (CAGA) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04572P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

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Product Overview

Description	Recombinant Helicobacter Pylori Cytotoxicity-Associated Immunodominant Antigen (CAGA) Protein (His) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P55980
Target Symbol	CAGA
Synonyms	cagA; cag26; cai; HP_0547Cytotoxicity-associated immunodominant antigen; 120 kDa protein; CAG pathogenicity island protein 26
Species	Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	MTNETIDQTRTPDQTQSQTAFDPQQFINNLQVAFIKVDNVVASFDPDQKPIVDKNDRDNRQAFDGISQLREEYSNKAIKNPTKKNQYFSDFIDKSNDLINKDNLIDVESSTKSFQKFGDQRYQIFTSWVSHQKDPSKINTRSIRNFMENIIQPPIPDDKEKAEFLKSAKQSFAGIIIGNQIRTDQKFMGVFDESLKERQEAEKNGGPTGGDWLDIFLSFIFNKKQSSDVKEAINQEPVPHVQPDIATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKF
Expression Range	1-294aa
Protein Length	Partial
Mol. Weight	35.6kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May be necessary for the transcription, folding, export, or function of the cytotoxin.
Database References	KEGG: heo:C694_02825 STRING: 85962.HP0547

Gene Functions References

The authors conclude that CagQ is a membrane protein in a type IV secretion system and is crucial for maintaining CagA expression and CagA-induced apoptotic effects. PMID: 29306012
The vacuolating cytotoxin autotransporter (vacA) c1 genotype, but not cytotoxicity-associated immunodominant antigen (cagA) status, might be the first important bacterial biomarker for predicting the cardia adenocarcinoma risk. PMID: 29036792
In this study, through quantitative analysis of the complex formation between CagA and PAR1b, the authors found that several CagA species have acquired elevated PAR1b-binding activity via duplication of the CagA multimerization motifs, while others have lost their PAR1b-binding activity. PMID: 27445265
Helicobacter pylori expressing cagA and vacA genes is prevalent in dyspeptic Ghanaian patients. PMID: 28655347
H. pylori infection significantly inhibits the cleavage of TRAF1 via a CagA-dependent mechanism, which would increase the relative amounts of full-length TRAF1 and exert an antiapoptotic effect on H. pylori-infected cells. PMID: 28082808
Data indicate that treatment of gastric epithelial cells with simvastatin reduced the level of cellular cholesterol and led to attenuation of translocation and phosphorylation of cytotoxin-associated gene A (CagA). PMID: 26730715
Studies suggest that cytotoxin-associated gene A (CagA)-negative non-virulent strains of Helicobacter pylori may be a potential risk factor of pancreatic cancer. PMID: 26390415
CagT plays an important role in the translocation of CagA into host epithelial cells. PMID: 23075784
CagA upregulated CIP2A expression and this upregulation effect was dependent on Src and Ras/mitogen-activated protein kinase kinase/extracellular signal-regulated kinase pathways. PMID: 19959630
In a stomach adenocarcinoma cell line, an increase in this protein correlated with the amount of the phosphorylated protein. PMID: 16278423
These data demonstrate that VacA can downregulate CagA's effects on epithelial cells, a novel molecular mechanism showing how H. pylori can avoid excessive cellular damage. PMID: 19046339

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.