Recombinant Human CD200R1L Protein (His Tag)

Name: Recombinant Human CD200R1L Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0786
Availability: InStock

Collections: Car-nk targets proteins, Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	AAT00538.1
Synonym	CD200R2, CD200RLa
Background	Cell surface glycoprotein CD2 receptor 2, also known as Cell surface glycoprotein CD2 receptor 1-like, Cell surface glycoprotein OX2 receptor 2, CD2 receptor-like 2, CD2Rla, CD2R1L and CD2R2, is a single-pass type I membrane protein which belongs to theCD2R family. CD2R1L / CD2R2. It contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. CD2 is a transmembrane protein delivering immunoregulatory signals after engagement of CD2R. A family of CD2Rs exist ( CD2R1, CD2R2, CD2R3, CD2R4 ) with different tissue expression and functional activity. In the presence of anti-CD2R2 / CD2R3 monoclonal antibodies (mAbs), bone-marrow cells cultured in the presence of (interleukin [IL]-4+granulocyte-macrophage colony-stimulating factor) differentiate into dendritic cells (DCs), which induce CD4+CD25+ Treg. Interaction between the relatively ubiquitously expressed molecule CD2 and one of its receptors, CD2R1, resulted in direct suppression of alloreactivity, engagement of alternate receptors led instead to altered differentiation of dendritic cells (DCs) from marrow precursors, which could in turn foster development of Foxp3(+) regulatory T cells. Unlike anti-CD2R1, anti-CD2R2 both promotes development of DCs with capacity to induce Treg and directly augments thymocyte production of Treg.
Description	A DNA sequence encoding the human CD200R1L (AAT00538.1) extracellular domain (Met1-Leu239) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gly 24
AA Sequence	Met1-Leu239
Molecular Weight	The recombinant human CD200R1L consists of 227 a.a. and predictes a molecular mass of 25.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD200R1L is approximately 45-60 kDa due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.