Recombinant Human CD24 Protein-VLP, Active

Beta LifeScience SKU/CAT #: BLK-02547P-100UG
Immobilized Human CD24 VLP at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 16.4ng/ml determined by ELISA.
Immobilized Human CD24 VLP at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 16.4ng/ml determined by ELISA.

Recombinant Human CD24 Protein-VLP, Active

Beta LifeScience SKU/CAT #: BLK-02547P-100UG
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description Recombinant Human CD24 Protein-VLP is expressed from HEK293.It contains Ser27-Gly59.
Accession P25063
Target Symbol CD24
Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043
Species Human
Expression System HEK293
Expression Range Ser27-Gly59
Mol. Weight The target protein has a predicted MW of 5.0 kDa.
Form Liquid
Formulation Supplied as 0.22um filtered solution in PBS (pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.
Endotoxin Less than 1EU per ug by the LAL method.
Activity Immobilized Human CD24 VLP at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 16.4ng/ml determined by ELISA. Contact us for detailed testing images.
Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping Shipped with dry ice.
Gene Background CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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