Recombinant Human CD40 Ligand/TNFSF5 Protein, Active

Name: Recombinant Human CD40 Ligand/TNFSF5 Protein, Active
Brand: Beta LifeScience
SKU: BLK-00247P-100UG
Availability: InStock

Human CD40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Car-nk targets proteins, Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant Human CD40 Ligand/TNFSF5 Protein is expressed from E.coli with His tag at the C-terminus.It contains Gln114-Leu261.
Purity	> 95% as determined by Tris-Bis PAGE
Accession	P29965
Target Symbol	CD40 Ligand/TNFSF5
Species	Human
Expression System	E. coli
Tag	C-His
Expression Range	Gln114-Leu261
Mol. Weight	The protein has a predicted MW of 17.15 kDa same as Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22 um filtered solution in PBS, 10% glycerol (pH 7.4).
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human CD40 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. Contact us for detailed testing images.
Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.