Recombinant Human CD51 & ITGB6 Heterodimer Protein

Name: Recombinant Human CD51 & ITGB6 Heterodimer Protein
Brand: Beta LifeScience
SKU: BLPSN-1037
Availability: InStock

Collections: Car-nk targets proteins, Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	P06756
Synonym	ITGAV &, ITGB6
Background	Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to theintegrin alpha chain family. ITGA5 contains 7FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.
Description	A DNA sequence encoding the extracellular domain (Met 1-Val992) of human ITGAV (P06756-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asn707) of human ITGB6 (P18564-1) was fused with a His tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human ITGAV&ITGB6 heterodimer was purified.
Source	HEK293
Predicted N Terminal	Phe 31 & Gly 22
AA Sequence	Met1-Asn707
Molecular Weight	The recombinant heterodimer of human ITGAV&ITGB6 comprises 1731 (1003+728) a.a. and has a calculated molecular mass of 190.4 (111.1+79.3) KDa.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling. ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1. Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation. ITGAV:ITGB3 act as a receptor for CD40LG.; (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for Adenovirus type C.; (Microbial infection) Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for Coxsackievirus A9 and B1.; (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8.; (Microbial infection) Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1.; (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1.; (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus.; (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, focal adhesion.
Protein Families	Integrin alpha chain family
Database References	HGNC: 6150 OMIM: 193210 KEGG: hsa:3685 STRING: 9606.ENSP00000261023 UniGene: Hs.436873

Gene Functions References

Binding of small molecule ligands and radiolabelled RGD peptides is modulated by expression and activation status of alphavbeta3 integrin. PMID: 28695371
There is no difference in the expression of Integrin alphavbeta3 between tumor samples from glioblastomas patients with methylated or unmethylated promoter regions in the (6)O-methylguanine methyltransferase (MGMT). Further analysis of the integrin subunits via ELISA from histologic sections revealed that there is no difference in integrin subunit expression between these patients. PMID: 29882028
These data suggested CD51 was a predictor for the prognosis of esophageal squamous cell carcinoma patients PMID: 30049512
This study shows that the D2A sequence of the UPAR induces cell growth through alphaVbeta3 integrin and EGFR. PMID: 29184982
Cyclin D1b markedly amplified integrin alphavbeta3 expression, which was further up-regulated under lipopolysaccharide stimulation. PMID: 30074214
differential expression of alphavbeta3 and alphavbeta6 was examined in MDA-MB-231, MDA-MB-468 and MCF-10A cells, which signify three different stages of breast cancer development from highly metastatic to non-tumorigenic stage. PMID: 29577899
High expression of Integrin alphaVbeta6 is an important cause of active proliferation and impaired apoptosis in cervical cancer. PMID: 28682441
The results of the present study suggest that the traditional Chinese formula WD3 may inhibit gastric tumor growth, potentially via the downregulation of integrin alphav beta3 and the inhibition of ERK1/2 phosphorylation in vivo PMID: 29152665
of AIM-cleavage and resulting functional modification could be the basis for designing safe and efficient AIM therapy for various diseases PMID: 27929116
show that sCD40L/alpha5beta1 interaction leads to platelet activation as evaluated in the human whole blood PMID: 26719354
Among Greek and Polish patients with intracerebral hemorrhage, the rs7565633 tag SNP of the ITGAV gene was independently associated with the risk of lobar ICH in a codominant model of inheritance. PMID: 27476161
Although Mn(2+) potently activates other integrins and increases affinity of alphaVbeta6 for pro-TGF-beta1 25- to 55-fold, it increases alphaVbeta8 affinity only 2- to 3-fold. PMID: 28484027
ITGAVITGAVITGAV PMID: 27363302
This work identifies that therapeutic targeting of integrins containing an alpha V integrin unit inhibits cancer progression and thus may be of clinical benefit. PMID: 28314844
this study uncovers a novel pathway by which the TGFbeta-activating integrin alphavbeta8 is expressed in the human intestine on dendritic cell subsets, which is upregulated in patients with inflammatory bowel disease PMID: 27782111
Integrin alphavbeta3 has a role in enhancing beta-catenin signaling in acute myeloid leukemia harboring Fms-like tyrosine kinase-3 internal tandem duplication mutations PMID: 27248172
BKCa has a role in promoting growth and metastasis of prostate cancer through facilitating the coupling between alphavbeta3 integrin and FAK PMID: 27233075
Inhibition of S. aureus ClfA binding to endothelial cell alphaV beta3 by cilengitide prevents endothelial dysfunction PMID: 27606892
Data show that sulfatide promoted integrin alphaV(ITGAV) expression and stimulated integrin alphaVbeta3 clustering and signaling in hepatocellular carcinoma (HCC) cells. PMID: 27145276
in shENO1 pancreatic ductal adenocarcinoma (PDA) cells, downregulation of proteins involved in cell-cell and cell-matrix adhesion include alpha v/beta 3 integrin PMID: 28086938
Low integrin alphaVbeta3 expression is associated with glioblastoma. PMID: 26918452
Data show that FGF2 mutants have potential as anti-angiogenic agents and useful tools for studying the role of integrin alphavbeta3 in FGF2 signalling. PMID: 28302677
VANGL2 interacts with Integrin alphaVbeta3 to regulate matrix metalloproteinase activity and cell adhesion to the extracellular matrix. PMID: 29097183
The results indicate that shear stress is an important mediator in endothelial progenitor cells expression of CD59 regulated by the extacellular matrix-integrin alphaVbeta3-F-actin pathway, which is a key factor in preventing membrane attack complex-mediated cell autolysis. PMID: 28943429
The mechanism of resistance of tongue squamous carcinoma cells Cal27 with de novo integrin alphavbeta3 expression to anticancer drugs was studied. In Cal27 cells integrin alphav heterodimers signal through pSrc(Y418) while this is not the case in integrin alphavbeta3-expressing cells. PMID: 27108184
data demonstrate that M. tuberculosis stimulation upregulates integrin alphaVbeta3 expression on monocytes, which upregulates secretion of MMP-1 and -10 on adhesion to the extracellular matrix; this leads to increased monocyte recruitment and collagenase activity, which will drive inflammatory tissue damage PMID: 28646039
The synergy of circulating factor suPAR and APOL1 G1 or G2 on alphavbeta3 integrin activation is a mechanism for CKD. PMID: 28650456
overexpression of syndecan-1 confers to B-LCs an increased capacity to migrate in response to Tat, owing to a switch from a CXCR4/G-protein/Rac to a syndecan-1/alphavbeta3/pp60src/pp125FAK signal transduction pathway that depends on the formation of a complex in which syndecan-1 interacts with Tat via its HS-chains, with alphavbeta3 via its core protein ectodomain and with pp60src via its intracellular tail PMID: 27819680
These results indicate that CD51 is a novel functional marker for colorectal CSCs which may provide an therapeutic target for the efficient elimination of colorectal CSCs. PMID: 27593923
promoting differentiation of luminal A breast cancer cells by signaling emanating from Int-alphavbeta3 can potentially promote 'normalization' of their malignant phenotype and may prevent the malignant cells from progressing. PMID: 27906177
Data show that dendritic cells (DCs) induced Th17 cell differentiation through miR-363/Integrin alphav/TGF-beta pathway in patients with rheumatoid arthritis (RA). PMID: 28376277
Myocardial alphavbeta3 integrin expression represents a marker of ongoing cardiac repair following acute myocardial infarction. PMID: 27927700
integrin alphaVbeta6 binds pro-TGF-beta1 in an orientation biologically relevant for force-dependent release of TGF-beta from latency PMID: 28117447
In vitro binding assays with purified components reveal that Tie-integrin recognition is direct, and further demonstrate that the receptor binding domain of the Tie2 ligand Ang-1, but not the receptor binding domain of Ang-2, can independently associate with a5b1 or aVb3. cooperative Tie/integrin interactions selectively stimulate ERK/MAPK signaling in the presence of both Ang-1 and fibronectin PMID: 27695111
The findings suggest that, in contrast with previous models, the ligand-binding site of integrin alphaVbeta3, binds to the constant region (helices A and B) of the EC2 domain of CD9, CD81, and CD151 antigens. PMID: 27993971
Antiendothelial alphavbeta3 antibodies are a major cause of intracranial bleeding in fetal/neonatal alloimmune thrombocytopenia. PMID: 27283740
Integrin alphav is required for local activation of latent TGFbeta, and the wound healing defect in the setting of integrin alphav loss was rescued by exogenous, active TGFbeta. PMID: 27295308
Taken together, our findings suggested that HMGB1 enhances tumor cell migration ability by activating alphavbeta3/FAK through TLR4/NF-kappaB signaling, leading to metastasis of NSCLC. PMID: 27769864
these results show that differences in stiffness/fluidity due to alphavbeta3 integrin expression or integrin activation by Mn(2+) might not simply be explained by the coupling of integrins to actin via focal adhesions. PMID: 27553273
Thus, the alphaV integrin subunit is important for varicella-zoster virus gB/gH-gL-mediated viral membrane fusion and infection. PMID: 27279620
The blockage of alphavbeta3 integrin inhibited the FAK-Src association and VEGFR activation, thus reducing tubulogenesis. PMID: 27420801
in some cell lines the cellular entry of human parechovirus 1(HPeV-1) is primarily mediated by the active form of alphaVbeta1 integrin without visible receptor clustering PMID: 27128974
The results implied that these five single nucleotide polymorphisms in the integrin alphavbeta3 gene were not associated with hemorrhagic fever with renal syndrome susceptibility or severity in Han Chinese individuals in Hubei Province. PMID: 28190175
Gastric cancer patients who were both alphavbeta6 and MMP-9 positive had a shorter overall survival. PMID: 27076771
The results of this study demonstrate that molecular dynamics of alphav integrin-GFP can be imaged in lung metastasis, which will allow further understanding of the role of alphav integrin in this process. PMID: 27466481
Periostin expression in human periodontal ligament fibroblasts promotes the migration of human mesenchymal stem cells through the alphavbeta3 integrin/FAK/PI3K/Akt pathway in vitro. PMID: 25900259
Results showed that CD51 expression in pancreatic cancer stroma is associated with enhanced tumor malignancy. PMID: 26846197
endothelial VEGFR-2 appeared to be slightly superior to endothelial alpha v ss 3 for differentiating benign from cancerous lesions. PMID: 26902100
Identify integrin alphavbeta3 as a receptor ofNC1 domain of collagen XIX. NC1(XIX) inhibits the FAK/PI3K/Akt/mTOR pathway, by decreasing the phosphorylation and activity of the major proteins involved in this pathway. PMID: 26621838
In this study, the long binding helix of this ligand was downsized to an enzymatically stable cyclic peptide endowed with sub-nanomolar binding affinity toward the alphavbeta6 receptor and remarkable selectivity against other integrins PMID: 26663660

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

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