Recombinant Human Claudin 4 Protein-VLP, Active

Name: Recombinant Human Claudin 4 Protein-VLP, Active
Brand: Beta LifeScience
SKU: BLK-00307P-100UG
Availability: InStock

The purity of Human Claudin 4 VLP is greater than 95% as determined by SEC-HPLC.

Collections: Recombinant proteins, Virus-like particle (vlp) proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant Human Claudin 4 Protein-VLP is expressed from HEK293.It contains Met1-Val209.
Purity	> 95% as determined by HPLC
Accession	O14493
Target Symbol	Claudin 4
Species	Human
Expression System	HEK293
Expression Range	Met1-Val209
Mol. Weight	The target protein has a predicted MW of 23.31 kDa.
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in PBS, 300mM L-Arginine (pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human Claudin 4 VLP at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Claudin 4 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. Contact us for detailed testing images.
Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	Claudin-4 (CLDN4) is a key component of tight junctions (TJs) in epithelial cells. CLDN4 is overexpressed in many epithelial malignancies and correlates with cancer progression. Changes in CLDN4 expression have been associated with epigenetic factors (such as hypomethylation of promoter DNA), inflammation associated with infection and cytokines, and growth factor signaling. CLDN4 helps to maintain the tumor microenvironment by forming TJs and acts as a barrier to the entry of anticancer drugs into tumors.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.