Recombinant Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G / RSV-G Protein (93% Homology) (His Tag)

Name: Recombinant Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G / RSV-G Protein (93% Homology) (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4950
Availability: InStock

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

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Product Overview

Tag	His
Host Species	RSV
Accession	P27022
Synonym	RSV Glycoprotein_G,
Background	Respiratory syncytial virus G protein is a protein produced by respiratory syncytial virus. It has been proposed as a target for a vaccine. RSV is an enveloped, negative-sense single-stranded RNA virus that belongs to the Pneumoviridae family. RSV virions have three proteins on the surface: the aforementioned F glycoprotein, the attachment glycoprotein (G), and the small hydrophobic protein. Full-length RSV G is a type II membrane protein with 30-40 O-linked glycans and 3-5 N-linked glycans that contribute ~60% of the glycoprotein's molecular weight. RSV G is a major target of the humoral immune response, and antibodies that target the central conserved region of G have been shown to neutralize both subtypes of RSV and to protect against severe RSV disease in animal models
Description	The glycoprotein G extracellular domain (Asn 66-Arg297) of human respiratory syncytial virus A (93% homologous with strain rsb1734) (P27022-1) was produced in HEK293 cells and fused with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Asn 66
AA Sequence	Asn 66-Arg297
Molecular Weight	The secreted recombinant human RSV-G comprises 242 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of highly glycosylation, the apparent molecular mass of RSV protein G is approximately 60-90 kDa in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the RSV G protein as determined by the LAL method
Formulation	This product was lyophilized from sterile PBS, pH 7.4
Stability	Recombinant RSV G antigens are stable for up to 1 year from date of receipt at -80°C
Applications	ELISA; immunogen; WB, etc.
Usage	For Research Use Only
Storage	Store RSV-G protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.