Recombinant Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1 / M1 Protein (His Tag)

Name: Recombinant Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1 / M1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-5018
Availability: InStock

Collections: Featured viral antigens molecules, Influenza a/b antigen, Recombinant proteins, Viral antigen

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Product Overview

Tag	His
Host Species	Influenza A H1N1
Accession	Q8BAC3
Description	The influenza A virus (A/Brevig- Mission/1/ 1918(H1N1)) matrix protein 1(Q8BAC3.1) (Met1-Lys252), termed as M1, was fused with a N-terminal His tag.
Source	E.coli
Predicted N Terminal	His
AA Sequence	Met1-Lys252
Molecular Weight	The recombinant M1 subunit of influenza A H1N1 consists of 268 amino acids and has a predicted molecular mass of 30 kDa. It migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
Purity	Greater than 90% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	This product was lyophilized from sterile 1mM EDTA, 50mM Tris, 50mM NaCl, 5% glycerol.
Stability	Recombinant antigens are stable for up to 1 year from date of receipt at -80°C
Applications	ELISA; immunogen; WB, etc.
Usage	For Research Use Only
Storage	Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.