Recombinant Moloney Murine Leukemia Virus Gag Polyprotein (GAG) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09904P
Greater than 90% as determined by SDS-PAGE.
Recombinant Moloney Murine Leukemia Virus Gag Polyprotein (GAG) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09904P
Collections: Featured viral antigens molecules, Hiv, Recombinant proteins, Viral antigen
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | Recombinant Moloney Murine Leukemia Virus Gag Polyprotein (GAG) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprotkb | P03332 |
| Target Symbol | GAG |
| Synonyms | gag; Gag polyprotein; Pr65gag; Core polyprotein) [Cleaved into: Matrix protein p15; MA); RNA-binding phosphoprotein p12; pp12); Capsid protein p30; CA); Nucleocapsid protein p10-Gag; NC-gag)] |
| Species | Moloney murine leukemia virus (isolate Shinnick) (MoMLV) |
| Expression System | E.coli |
| Tag | N-6His-SUMO |
| Target Protein Sequence | PLRAGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAGLQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTEDEQKEKERDRRRHREMSKLL |
| Expression Range | 216-478aa |
| Protein Length | Partial |
| Mol. Weight | 46.6kDa |
| Research Area | Microbiology |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
| Target Function | Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.; Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.; Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.; Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.; Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization. |
| Subcellular Location | [Gag polyprotein]: Virion. Host cell membrane; Lipid-anchor. Host late endosome membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p15]: Virion.; [Capsid protein p30]: Virion.; [Nucleocapsid protein p10-Gag]: Virion.; [RNA-binding phosphoprotein p12]: Host cytoplasm. |
| Database References | KEGG: vg:1491870 |
Gene Functions References
- The structure of the minimal sequence of the murine leukemia virus p12 protein necessary for the host chromatin binding was elucidated. PMID: 27707926
- Phosphorylation requirement of murine leukemia virus p12 virus p12 protein necessary for the host chromatin binding was elucidated. PMID: 27707931
- The N-terminal intracellular domain of the glycoGag is responsible for its effect on HIV-1 infectivity. PMID: 24403584
- Authors found that mature Gag proteins MA and TM were bound to the immature core consisting of the Gag polyprotein (PrGag) and viral RNA (vRNA). PMID: 23643491
- in presence of nucleocapsid (NC) chaperone, RNA dimerization occurred rapidly through a single structural intermediate; NC interacted primarily with guanosine residues; results show RNA chaperones can simplify RNA folding landscapes by weakening intramolecular interactions involving guanosine PMID: 23470731
- selective packaging of dimeric RNA is explained by the nucleic acid binding domain derived from Gag binding within the virus to the sequence UCUG-UR-UCUG PMID: 20974908
- results suggest a cooperative effect of p12 and capsid during the early events of MLV replication PMID: 15767417
- interaction of Gag with Tsg101 and Alix favors budding from the plasma membrane and relieves a requirement for ubiquitination by Nedd4 PMID: 15908698
- results indicate that glycosylated gag protein is important for M-MuLV replication in vivo and in vitro and that the protein may be involved in a late step in viral budding or release PMID: 17267509
- Abl protein contribute to proper localization by playing a dominant role in trafficking of the v-Abl molecule PMID: 18367522
- Basal budding and replication of the murine leukemia virus are independent of the gag L domains PMID: 18667521
