Recombinant Mouse C-Type Lectin Domain Family 4 Member K (CD207) Protein (His)

Name: Recombinant Mouse C-Type Lectin Domain Family 4 Member K (CD207) Protein (His)
Brand: Beta LifeScience
SKU: BLC-11112P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: Car-nk targets proteins, Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Mouse C-Type Lectin Domain Family 4 Member K (CD207) Protein (His) is produced by our Mammalian cell expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q8VBX4
Target Symbol	CD207
Synonyms	Cd207; Clec4kC-type lectin domain family 4 member K; Langerin; CD antigen CD207
Species	Mus musculus (Mouse)
Expression System	Mammalian cell
Tag	N-10His
Target Protein Sequence	QAVFYPRLMGKILDVKSDAQMLKGRVDNISTLGSDLKTERGRVDDAEVQMQIVNTTLKRVRSQILSLETSMKIANDQLQILTMSWGEVDSLSAKIPELKRDLDKASALNTKVQGLQNSLENVNKLLKQQSDILEMVARGWKYFSGNFYYFSRTPKTWYSAEQFCISRKAHLTSVSSESEQKFLYKAADGIPHWIGLTKAGSEGDWYWVDQTSFNKEQSRRFWIPGEPNNAGNNEHCANIRVSALKCWNDGPCDNTFLFICKRPYVQTTE
Expression Range	63-331aa
Protein Length	Partial
Mol. Weight	34.8 kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.
Subcellular Location	Membrane; Single-pass type II membrane protein.
Database References	KEGG: mmu:246278 STRING: 10090.ENSMUSP00000040746 UniGene: Mm.136079
Tissue Specificity	Expressed by Langerhans cells. Expressed in dendritic cells and by scattered cells in lymph nodes and spleen. Also detected in some non-lymphoid tissues such as lung, liver and heart.

Gene Functions References

Binding analysis between purified long and short forms of langerin and a series of keratin sulfate disaccharide components shows that the binding critically requires the 6-sulfation of Gal and that the long form of langerin displays higher affinity than the short form. PMID: 29631057
Bacterial Polysaccharide Specificity of the Pattern Recognition Receptor Langerin Is Highly Species-dependent PMID: 27903635
Data show that Candida albicans and Candida tropicalis preferentially localized in (and persisted within) a sub- population of Peyer's patch Langerin-positive dendritic cells. PMID: 26386376
Langerin is expressed by the XCR1(+) "DC1" population of mice. PMID: 25516751
Langerin+ dermal dendritic cells appear to be site- and strain-specific PMID: 24629018
model of epicutaneous ovalbumin sensitization inducing an inflammatory skin resembling atopic dermatitis (AD) to explore the role of CD207 in the pathogenesis of AD PMID: 23117802
Langerin-positive dendritic cells appear to be the predominant, though not exclusive, population responsible for in vivo transport of anti-DEC-205 antibodies from the skin to the draining lymph nodes in steady state and inflammation. PMID: 22291181
Data demonstrated that CD207(+) CD8alpha(+) thymic DCs do not share a common origin with T cells but originate from intrathymic precursors. PMID: 21630253
The normal corneal epithelium is endowed with CD11c(+) Langerin+ cells that are LCs, whereas the stroma is endowed with a separate population of (non-LC) Langerin+ DCs. PMID: 21482644
Langerhan cells and (Langerin(+)) dermal dendritic cells thus seem to have a redundant function in regulating contact hypersensitivity PMID: 20703247
molecular cloning and expression of Langerin transcripts in dendritic cells PMID: 11978773
Developmentally regulated expression of Langerin isoforms provides a mechanism by which Langerin involvement in antigen uptake and processing could be regulated. PMID: 15191546
CD207 has a role in maintaining Birbeck granules but not in Langerhans cell function PMID: 15601833
Langerin may have an unanticipated role in cell-matrix interactions that modulate LC development, localization, or function PMID: 16557233
CD207 antigens manifest a high degree of colocalization around the nucleus of the Langerhans cells. PMID: 16941665
langerin(+) DCs patrol the dermis in the steady state and migrate to the skin DLNs charged with skin antigens PMID: 18086862
dermal Langerin(+) dendritic cells (DCs) are radiosensitive and display a distinct cell surface phenotype PMID: 18086865
Depletion of langerin(+) cells impaired intestinal IgA Ab responses after Topical transcutaneous immunization PMID: 18354155
Langerin(+) cells do promote T cell responses to skin Ags PMID: 18354196
In a knockin-mouse model of the immune response against Leishmania major parasites, langerin-expressing dendritic cells are, in general, dispensable for an efficient adaptive immune response against L. major parasites. PMID: 19124720
Despite a marginal zone location, CD207(+) DCs lacked identifying molecules for 3 different types of macrophages, localized in proximity and, in contrast to macrophages, marginal zone DCs were poor scavengers of soluble and particulate substrates PMID: 19168629
Langerhans cells(LC) and langerin(+) dermal dendritic cells(DC) represent distinct DC subsets that have specialized functions and that LC are important immunoregulatory cells PMID: 19218433

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.