Recombinant Mouse IL6 Protein

Name: Recombinant Mouse IL6 Protein
Brand: Beta LifeScience
SKU: BLPSN-2920
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines and growth factors, Interleukins and receptors, Recombinant interleukin-6 (il-6) proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Mouse
Accession	P08505
Synonym	Il-6
Background	Interleukin-6 (IL-6) is a multifunctional alpha-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 13 (gp13) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the mouse IL6 (P08505) (Phe25-Thr211) was expressed and purified.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Phe25-Thr211
Molecular Weight	The recombinant mouse IL6 consists of 188 a.a. and predicts a molecular mass of 21.9 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	1. Measured by its ability to bind biotinylated rat IL6ST-mFch in a functional ELISA.2. Measured by its ability to bind biotinylated mouse IL6ST-Fc2h in a functional ELISA.3. Measured by its ability to bind biotinylated mouse IL6ST-His in a functional ELISA.4. Measured by its ability to bind biotinylated human IL6RB-Fch in a functional ELISA.5. Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
Subcellular Location	Secreted.
Protein Families	IL-6 superfamily
Database References	KEGG: mmu:16193 STRING: 10090.ENSMUSP00000026845 UniGene: Mm.1019
Tissue Specificity	Expressed by dendritic cells and macrophages. Expressed by activated follicular B cells. Abundantly expressed in the central nervous system (CNS), particularly the hypothalamic region.

Gene Functions References

These results suggest that TCF21 contributes to the proinflammatory environment in VIS fat depots and to active ECM remodeling of these depots by regulating IL6 expression and MMP-dependent ECM remodeling in a spatiotemporally coordinated manner. PMID: 29540474
Notch signaling regulates cell density-dependent apoptosis through IL-6/STAT3-dependent mechanism. PMID: 30249464
Obesity-induced IL-6 shifts macrophage polarization towards tumor-promoting macrophages that produce the CCL-20 in the colitis-associated colorectal cancer (CAC) microenvironment. CCL-20 promotes CAC progression by recruiting CCR6-expressing B-cells and gammadelta T cells via chemotaxis. PMID: 29695802
In glioblastoma, colony-stimulating factor-1 and angiocrine IL-6 induce robust arginase-1 expression and macrophage alternative activation, mediated through peroxisome proliferator-activated receptor-gamma-dependent transcriptional activation of hypoxia-inducible factor-2alpha. PMID: 29422647
microbiota provide a homeostatic role for epithelial barrier function through regulation of intraepithelial lymphocytes -derived IL-6 PMID: 28812548
Ischemia augments alloimmune injury through IL-6-driven CD4+ alloreactivity. PMID: 29410442
Results suggest that IL-6 contributes to limit lipid metabolic disorder, cardiac hypertrophy, fibrosis, inflammation and myocardium lipotoxicity during HFD-induced obesity. PMID: 28844956
CD37 may protect against IgA nephropathy by inhibition of the IL-6 pathway. PMID: 29551516
IL-6/Stat3 signaling drives a transcriptional program of antimicrobial gene expression in infected urothelium, with key roles in limiting epithelial invasion and ascending infection. PMID: 29475562
IL-6 and STAT3 have roles in potentiating FGF19-driven hepatocellular carcinoma (HCC) in mice; this finding may have translational relevance in HCC pathogenesis PMID: 28508871
IGF-1R signalling contributes to T cell dependent inflammation in arthritis. Inhibition of IGF-1R on the level of insulin receptor substrates alleviates arthritis by restricting IL6-dependent formation of Th17 cells and may open for new treatment strategies in rheumatoid arthritis. PMID: 28583713
The present study demonstrates for the first time that IL-6 trans-signaling is involved in the pathomechanisms of compromised fracture healing after severe injury, whereas IL-6 classic signaling rather mediates pro-regenerative effects augmenting bone regeneration PMID: 29497762
persistent stimulation with titanium particles may lead to a consistent release of TNF-alpha and IL-6 via SPHK-2 activity, which may lead to aseptic implant loosening PMID: 29728804
IL-6-STAT3 signaling facilitates TRIM28 binding to the Il17-Il17f locus, and this process is required for epigenetic activation and high-order chromosomal interaction in autoimmune experimental encephalomyelitis. PMID: 29651155
IL-6 induces utrophin expression through NRG-1/ErbB pathway in dystrophic myotubes. PMID: 27988307
IL-6 overexpression could enhance cardiomyocyte proliferation and relevant protein expression in mice myocardium, thus promoting cardiac regeneration. PMID: 29966974
data revealed that IL-6 regulates the excessive release of NO through IL-1beta inhibition and determines the establishment of an M2 macrophage profile within infected heart tissue. PMID: 28087471
IL-6 plays a role in consolidation process. PMID: 29619678
IL-6 expands dendritic cell and monocytic populations and ultimately leads to a robust T-cell driven immune response in Complete Freund's Adjuvant immunized mice. PMID: 28474508
These results suggested that the thrombinstimulated synthesis of IL6 was limited by HSP90 in osteoblasts, and that the effects of HSP90 were exerted at the point between Rhokinase and p38 MAPK. PMID: 30066835
Results suggest that interleukin 6 (IL-6) may be exploited for lung repair during influenza infection. PMID: 28262742
YY1 was progressively up-regulated in BV2 microglial cells stimulated with lipopolysaccharide (LPS), which was dependent on the transactivation function of nuclear factor kappa B (NF-kappaB). Furthermore, YY1 knockdown notably inhibited LPS-induced the activation of NF-kappaB signaling and interleukin-6 (IL-6) expression in BV-2cells. PMID: 29803672
The authors conclude that Mycobacterium tuberculosis ESAT-6 stimulates macrophage IL-6 production through STAT3 activation. PMID: 28106119
IL-6/soluble IL-6R differentially regulate RANKL-induced osteoclast differentiation and activity through modulation of NF-kappaB, ERK and JNK signaling pathways. PMID: 28128332
These results suggest that the inhibition of IL6/STAT3 signaling is a potential mechanism by which PZH is used in the treatment of ulcerative colitis. PMID: 29845215
CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID: 29901197
results suggest that IL-6 gene requires up-regulation of phospho-JAK2/STAT3, PACAP, and PAC1R and down-regulation of the TNF-alpha gene to modulate its anticonvulsive/neuroprotective potential PMID: 29673861
Toll-like receptor 4 (TLR4) requires physical and functional association with Fcalpha-mu protein (Fcalpha/muR) for its oligomer formation and interleukin-6 (IL-6) production from marginal zone (MZ) B cells. PMID: 27146354
TRYP improves the health condition of mice with DSS induced colitis by regulating the TNF-α/NF-κBp65 and IL-6/STAT3 signaling pathways via inhibiting the degradation of IκBα and the phosphorylation of STAT3. PMID: 29724065
Our results demonstrate that IL-6 activation in placenta is required for relaying inflammatory signals to the fetal brain and impacting behaviors and neuropathologies relevant to neurodevelopmental disease. PMID: 27838335
An IL-6 infusion model can initiate macrophage accumulation as well as aortic dilation, and under conditions of elevated tension, this proinflammatory cytokine can be produced by aortic vascular smooth muscle cells. PMID: 29107003
miR-155 seems to target Est-1 and induces ulcerative colitis via the IL-23/17/6-mediated Th17 pathway. PMID: 28888763
IL-6 and aging are involved in regulation of PPARalpha and PGC-1alpha expression and may influence the mitochondrial function. PMID: 29173012
CGRPinduced IL6 mRNA expression was mediated by mmu_circRNA_007893. PMID: 29039515
The results of this study indicated that persistently increased levels of IL-6 can lead to alterations in mTOR regulation of L-LTP. PMID: 29104031
These data demonstrate that the Pb18 strain of Paracoccidioides brasiliensis is able to activate the transcription of Notch1 receptor in J774 macrophages. Activation of this receptor with also activation of TLR 4 (via LPS) induces IL-6 production, which favors the pathogenesis. PMID: 28600728
IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID: 28453771
IL 6 and TGF beta perform essential role in cerebral malaria pathogenesis by modulating the level of glial cell induced neuroinflammation. PMID: 28803696
These results suggest that glucocorticoids' effects on adipose tissue immune response, both in a pro- and an anti-inflammatory manner, depend on the nutritional status. PMID: 29847081
EMMPRIN inhibited bFGF-induced IL-6 secretion by reducing the p65 subunit phosphorylation, it might be concluded that bFGF and EMMPRIN crosstalk in their respective signaling pathways. PMID: 29104472
pathologic levels of IL-6 in the periphery may play a role in the development of seizures when viral replication within the brain is limited following infection with a variant of Theiler's murine encephalomyelitis virus that does not replicate within the parenchyma of the brain. PMID: 28741149
TIARP independently down-regulated CXCL2 and IL-6 production by fibroblast-like synoviocytes, and the expression of chemokine receptors (CXCR1 and CXCR2) in neutrophils, with resultant reduction of neutrophil migration into arthritic joints. PMID: 27995997
these findings revealed a novel and unexpected role of IL-6 in ameliorating acute liver injury via regulating inflammatory responses in hepatic macrophages PMID: 28822324
increased circulating levels of IL-6 perturb the redox signaling cascade, even prior to the necrotic stage, leading to severe features and progressive nature of muscular dystrophy. PMID: 28845212
LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14 PMID: 28637991
role in keratinocyte migration and proliferation through modulation of TGF-betaR expression and function PMID: 27892604
The in vitro findings suggest that GTS-21-induced IL-6 release from muscle is mediated via alpha7AChRs upstream of Stat-3 and -5 pathways and is associated with myonuclear accretion, possibly via MyoD and Pax7 expression. PMID: 28282360
Burn serum caused muscle cell death associated with increased mitochondrial fission and functional impairment. This alteration was alleviated with IL-6 antibody treatment, suggesting the cytokine plays a role in mitochondrial changes in muscle after systemic injury. PMID: 28181922
This study demonstrates that obesity-associated inflammation and metabolic disturbances depend on interleukin-6/Stat3-dependent formation of a distinct natural killer population, which may provide a target for the treatment of obesity, metaflammation-associated pathologies, and diabetes. PMID: 28683285
In the CNS, LPS administration had the greatest effect on IL-6 and LPS increased IL-6 mRNA expression only in non-neuronal cells. PMID: 28456715

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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