Recombinant Rat Syndecan-1 / SDC1 / CD138 Protein (Fc Tag)

Name: Recombinant Rat Syndecan-1 / SDC1 / CD138 Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-4387
Availability: InStock

Collections: Car-nk targets proteins, Cluster of differentiation (cd) proteins, Featured cd protein molecules, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Rat
Accession	P26260
Background	Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.
Description	A DNA sequence encoding the rat SDC1 (P26260) (Met1-Lys253) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Gln 23
AA Sequence	Met1-Lys253
Molecular Weight	The recombinant rat SDC1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 472 a.a. and has a predicted molecular mass of 51.2 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA. Immobilized human PTN at 10 ug/ml (100 ul/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 ug/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 ug/ml (100 ul/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.4-1.1 ug/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Subcellular Location	Membrane; Single-pass type I membrane protein. Secreted. Secreted, extracellular exosome.
Protein Families	Syndecan proteoglycan family
Database References	KEGG: rno:25216 STRING: 10116.ENSRNOP00000008582 UniGene: Rn.11176

Gene Functions References

Plasma syndecan-1 and heparan sulfate correlate with microvascular glycocalyx degradation in hemorrhaged rats after different resuscitation fluids. PMID: 27037369
down-regulation of Syndecan-1 could inhibit VEGF-VEGFR-2 signaling through regulating internalization of VEGFR-2. PMID: 27075925
expression upregulated two months after renal transplantation PMID: 25972509
data suggest that after renal transplantation loss of hepatic HS together with increased syndecan-1 shedding hampers lipoprotein binding and uptake by the liver contributing to dyslipidemia PMID: 25154787
analysis of the molecular events that drive endocytosis of syndecan-1, a raft-dependent receptor and identification of its novel endocytic motif, MKKK PMID: 23525115
The abnormal expression of HSPG may be an important molecular mechanism that leads to the occurrence and development of proteinuria in adriamycin-induced nephropathy rats. PMID: 20506637
role in regulatory mechanism of apoptosis PMID: 11979781
Syndecan-1 mRNA expression is not modulated by FSH as it would result from the antagonistic effects of increased intracellular cAMP and intracellular calcium levels. PMID: 12135485
The major injury-related change, seen in injured brain and cultured glia, was an increase in 2-O-sulphated HS and increased syndecan-1, suggesting novel approaches to modulating scar formation. PMID: 18279312

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.