Recombinant RVFV NP (Rift Valley Fever Virus)

Name: Recombinant RVFV NP (Rift Valley Fever Virus)
Brand: Beta LifeScience
SKU: BLIT-0654
Availability: InStock

Collections: Featured viral antigens molecules, Other viral antigens, Recombinant proteins, Viral antigen

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Product Overview

Tag	His
Description	Recombinant RVFV NP (Rift Valley Fever Virus) was expressed from 293 cell culture; C-terminal His tagged NP (RVFV) protein (a.a. 1-245) (Genebank Accession No: P21700).
Source	HEK293
AA Sequence	a.a. 1-245
Purity	> 95%
Formulation	Each vial contains 100 µg of purified protein (1 mg/ml) in PBS.
Applications	WB standard, antibody ELISA, immunogen, etc
Usage	For Research Use Only
Storage	The virus antigen is stable for a month at 4°C. It should be stored at -20°C from the date of shipment. Please avoid repeated freeze thaw cycles.

Target Details

Target Function	Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. After replication, the nucleocapsid is recruited to the host Golgi apparatus by glycoprotein Gn for packaging into virus particles.
Subcellular Location	Virion. Note=Internal protein of virus particle.
Protein Families	Phlebovirus nucleocapsid protein family

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.