Recombinant Sars-Cov-2 Nucleoprotein (N) Protein (His), Active

Name: Recombinant Sars-Cov-2 Nucleoprotein (N) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05804P
Availability: InStock

Greater than 93% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Mouse Monoclonal Antibody, the EC 50 of N Mouse Monoclonal Antibody is 3.246-4.503 ng/ml. Biological Activity Assay

Activity Measured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Recombinant Antibody, the EC 50 of N Recombinant Antibody is 2.825-4.124 ng/ml. Biological Activity Assay

Collections: Coronaviruses, Featured viral antigens molecules, Recombinant proteins, Recombinant proteins fall special offers - active proteins, Recombinant proteins fall special offers - full-length proteins, Viral antigen

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Product Overview

Description	Recombinant Sars-Cov-2 Nucleoprotein (N) Protein (His), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 93% as determined by SDS-PAGE.
Activity	1. Measured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Mouse Monoclonal Antibody, the EC 50 of N Mouse Monoclonal Antibody is 3.246-4.503 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized N ) at 2 μg/ml can bind N Recombinant Antibody, the EC 50 of N Recombinant Antibody is 2.825-4.124 ng/ml.
Uniprotkb	P0DTC9
Target Symbol	N
Synonyms	N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MSDNGPQNQRNALRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Expression Range	1-419aa(P13L)
Protein Length	Full Length
Mol. Weight	49.7 kDa
Research Area	Microbiology
Form	Lyophilized powder
Buffer	Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location	Virion. Host cytoplasm.
Protein Families	Betacoronavirus nucleocapsid protein family

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.