Recombinant SARS-COV-2 Spike RBD (N501Y) Protein, Active

Name: Recombinant SARS-COV-2 Spike RBD (N501Y) Protein, Active
Brand: Beta LifeScience
SKU: BLK-02341P-100UG
Availability: InStock

SARS-COV-2 Spike RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Recombinant proteins

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Product Overview

Description	Recombinant SARS-COV-2 Spike RBD (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y).
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	QHD43416.1
Target Symbol	Spike RBD (N501Y)
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Species	SARS-COV-2
Expression System	HEK293
Tag	C-His
Expression Range	Arg319-Phe541(N501Y)
Mol. Weight	The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.74nM as determined in SPR assay (Biacore T200). Contact us for detailed testing images.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.