Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Active

Name: Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein, Active
Brand: Beta LifeScience
SKU: BLK-01662P-100UG
Availability: InStock

Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Fc receptors, High-quality recombinant proteins

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Product Overview

Description	Recombinant Biotinylated Human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide.
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC
Target Symbol	HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer
Synonyms	MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
Species	Human
Expression System	HEK293
Tag	C-His-Avi
Expression Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide
Mol. Weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22 um filtered solution in 20mM PB, 500mM NaCl (pH 8.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Anti-HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-A02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.25ug/ml determined by ELISA. Contact us for detailed testing images.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.