Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein, Active

Name: Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein, Active
Brand: Beta LifeScience
SKU: BLK-00124P-100UG
Availability: InStock

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: Fc receptors, Recombinant proteins

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Product Overview

Description	Recombinant Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL
Target Symbol	HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer
Species	Human
Expression System	E. coli
Tag	C-His-Avi
Expression Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and RMFPNAPYL peptide
Mol. Weight	The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 300mM NaCl, 10%Glycerol (pH 8.0).
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. Contact us for detailed testing images.
Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.