Magic High-Fidelity DNA Polymerase

Name: Magic High-Fidelity DNA Polymerase
Brand: BetaLifeSci
SKU: ENY-121
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Magic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. In addition, Magic High-Fidelity DNA Polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases. Magic High-Fidelity DNA Polymerase has 5'→3' polymerase and 3'→5' exonuclease (proofreading) activity, but no 5'→3' exonuclease activity. The strong 3'→5' exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends. DNA fragments generated with Magic High-Fidelity DNA Polymerase may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity DNA Polymerase are blunt-ended, but may be 3'-dA-tailed for cloning into TA cloning vectors.
Source	An E.coli strain carries the Magic High-Fidelity DNA Polymerase gene.
Applications	High Fidelity PCR, High throughput PCR , Routine PCR
Size	100 U / 1000 U / 5000 U
Concentration	2,000 U/ml
Components	Magic High-Fidelity DNA Polymerase (2,000 U/ml); 5X Magic High-Fidelity DNA Polymerase HF Buffer; 5X Magic High-Fidelity DNA Polymerase GC Buffer; MgCl2 Solution
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C
Usage	For Research Use Only
Storage	Storage Conditions: 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 1X stabilizers, pH 7.4 @ 25°C. Store the Magic High-Fidelity DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.