MagicFast Pfu 2X PCR Master Mix with Dye

Name: MagicFast Pfu 2X PCR Master Mix with Dye
Brand: BetaLifeSci
SKU: ENY-117
Availability: InStock

Collections: Featured enzyme molecules, Tool enzymes & other reagents

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Pfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. MagicFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. MagicFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ~2 kb/min), high fidelity and strong anti-interference ability. Consequently, MagicFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. MagicFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. MagicFast Pfu 2X PCR Master Mix is an optimized ready-to-use solution containing MagicFast Pfu DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
Applications	High Fidelity PCR, PCR , Routine PCR
Size	100 rxn / 500 rxn
Concentration	2x
Components	MagicFast Pfu 2X PCR Master Mix with Dye
Usage	For Research Use Only
Storage	Store the MagicFast Pfu 2X PCR Master Mix with dye at -20°C. Please avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.