MagicScript II Reverse Transcriptase
BetaLifeSci
SKU/CAT #: ENY-125
MagicScript II Reverse Transcriptase
BetaLifeSci
SKU/CAT #: ENY-125
Collections: Featured enzyme molecules, Tool enzymes & other reagents
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
| Description | MagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb. |
| Source | The gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H-) is expressed in E. coli and purified to near homogeneity. |
| Applications | cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis , PCR, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis |
| Size | 4000 U / 10000 U |
| Concentration | 200,000 U/ml |
| Components | MagicScript II Reverse Transcriptase (200,000 U/ml); 100 mM DTT (10X); 5X First-Strand Buffer |
| Unit Definition | One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template. |
| Usage | For Research Use Only |
| Storage | Storage Conditions: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C. Store the MagicScript II Reverse Transcriptase at -20°C. Please avoid repeated freeze-thaw cycles. |
