Recombinant PE-Labeled Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein

Name: Recombinant PE-Labeled Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein
Brand: Beta LifeScience
SKU: BLK-00380P-100UG
Availability: InStock

Collections: Fc receptors, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Recombinant PE-Labeled Human HLA-A02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human HLA-A02:03&B2M&AFP (FMNKFIYEI) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide.
Accession	AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI
Target Symbol	HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer
Species	Human
Expression System	HEK293
Tag	C-His-Avi
Expression Range	Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in PBS, 0.2% BSA (pH 7.4).
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Valid for 6 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.