Recombinant Canine FGF14 Protein

Name: Recombinant Canine FGF14 Protein
Brand: Beta LifeScience
SKU: BLPSN-2035
Availability: InStock

Collections: Cytokines and growth factors, Enzymes, Growth factors and receptors, Kinase, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Tag	N/A
Host Species	Canine
Accession	XP_003363267.1
Synonym	Fibroblast growth factor 14, FGF-14, Fibroblast growth factor homologous factor 4, FHF-4, FGF14, FHF4, SCA27, bA397O8.2.
Background	Fibroblast Growth Factor 14 (FGF14) belongs to the fibroblast growth factor (FGF) family. FGFfamily members have broad mitogenic and cell survival activities, and are involved in a various biological processes, including embryonic development, cell growth
Description	A DNA sequence encoding the canine FGF14 (Lys64-Thr252)was expressed with a N-terminal Met.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Lys64-Thr252
Molecular Weight	The recombinant canine FGF14 comprises 190 amino acids and has a predicted molecular mass of 21.1 kDa. The apparent molecular mass of the protein is approximately 21 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Immobilized canine FGF14 at 10 ug/ml (100 ul/well) can bind human FGFR4-Fc with a linear range of 0.16-2.5 ug/ml.
Formulation	Recombinant Canine FGF14 Protein was lyophilized from sterile PBS, pH 7.4.
Stability	Recombinant Canine FGF14 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.