Recombinant Cynomolgus PDGF-B Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3710
Recombinant Cynomolgus PDGF-B Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3710
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Cynomolgus |
Accession | G7PFK7 |
Background | Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT.So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor. |
Description | A DNA sequence encoding the cynomolgus PDGFB (EHH65851.1) (Ser82-Thr190) was expressed with the Fc region of mouse IgG1 at the N-terminus. |
Source | HEK293 |
Predicted N Terminal | Asp |
AA Sequence | Ser82-Thr190 |
Molecular Weight | The recombinant cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 a.a. and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His at 10 ug/ml (100 ul/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |