Recombinant Horse Interleukin-1 Beta Protein (IL1B), Active
Beta LifeScience
SKU/CAT #: BLC-05352P
Recombinant Horse Interleukin-1 Beta Protein (IL1B), Active
Beta LifeScience
SKU/CAT #: BLC-05352P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Horse Interleukin-1 Beta Protein (IL1B), Active is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 95% as determined by SDS-PAGE and HPLC. |
Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to a specific activity of >5.0x10 7 IU/mg. |
Uniprotkb | Q28386 |
Target Symbol | IL1B |
Synonyms | IL1BInterleukin-1 beta; IL-1 beta |
Species | Equus caballus (Horse) |
Expression System | E.coli |
Tag | Tag-Free |
Complete Sequence | AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA |
Expression Range | 116-268aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 17.3 kDa |
Research Area | Immunology |
Form | Lyophilized powder |
Buffer | Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 0.1 Tween-80 |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
Subcellular Location | Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted. |
Protein Families | IL-1 family |
Database References |
Gene Functions References
- Increased PGE2 production led to reduction in 5-LO products in LPS-treated equine whole blood via IL-1b. PMID: 24530239
- Results suggested that chemokine expression by cultured equine BECs following exposure to pulmonary hemorrhage conditions may contribute to the development of inflammatory airway disease in horses. PMID: 22280393
- IL-1beta-induced up-regulation of matrix metalloproteinase 13 mRNA was blocked by all concentrations of geldanamycin tested PMID: 17599753
- This study examined effects of in vitro exposure to solutions of hay dust, lipopolysaccharides, or beta-glucan on cytokine expression in pulmonary mononuclear cells isolated from healthy horses and horses with recurrent airway obstruction. PMID: 18052742
- The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861
- The acute pulmonary neutrophilia characteristic of recurrent airway obstruction was not associated with an increase in expression of chemokines in pulmonary mononuclear cells from disease-susceptible horses. PMID: 19795943