Recombinant Human 2B4 / CD244 Protein (His Tag)

Name: Recombinant Human 2B4 / CD244 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0017
Availability: InStock

Collections: Cluster of differentiation (cd) proteins, Co-stimulatory receptors, Featured cd protein molecules, Featured immune checkpoint protein molecules, Immune checkpoint proteins, Others immune checkpoint proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	NP_057466.1
Synonym	CD244, 2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 molecule
Background	CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene.[5] It is also known as Natural Killer Cell Receptor 2B4.This gene encodes a cell surface receptor expressed on natural killer cells (NK cells) (and some T cells) mediating non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CD244 can also be expressed on non-lymphocytes such as eosinophils, mast cells and dendritic cells.
Description	A DNA sequence encoding the extracellular domain (Met 1-Arg 221) of human 2B4 (NP_057466.1) was produced, with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Cys 22
AA Sequence	Met 1-Arg 221
Molecular Weight	The recombinant 2B4 comprises 211 amino acids and predicts a molecular mass of 23.8 kDa. As a result of glycosylation, the rh 2B4 protein migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
Purity	Greater than 95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized human 2B4 at 2 ug/ml (100 ul/well) can bind human CD48. The EC50 of human CD48 is 0.39 ug/ml.
Formulation	Lyophilized from sterile PBS, pH 7.4
Stability	Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C
Usage	For Research Use Only
Storage	Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.