Recombinant Human Angiopoietin-Related Protein 2 (ANGPTL2) Protein (His-SUMO)

Name: Recombinant Human Angiopoietin-Related Protein 2 (ANGPTL2) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-04429P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ANGPTL2.

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Product Overview

Description	Recombinant Human Angiopoietin-Related Protein 2 (ANGPTL2) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9UKU9
Target Symbol	ANGPTL2
Synonyms	AI593246; Angiopoietin like 2; Angiopoietin related protein 2 ; Angiopoietin-like protein 2; Angiopoietin-related protein 2; ANGL2_HUMAN; Angptl2; Arp2; AW260363; HARP; MGC8889; UNQ170/PRO196
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	QEDGFEGTEEGSPREFIYLNRYKRAGESQDKCTYTFIVPQQRVTGAICVNSKEPEVLLENRVHKQELELLNNELLKQKRQIETLQQLVEVDGGIVSEVKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNALELSQLENRILNQTADMLQLASKYKDLEHKYQHLATLAHNQSEIIAQLEEHCQRVPSARPVPQPPPAAPPRVYQPPTYNRIINQISTNEIQSDQNLKVLPPPLPTMPTLTSLPSSTDKPSGPWRDCLQALEDGHDTSSIYLVKPENTNRLMQVWCDQRHDPGGWTVIQRRLDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTMEDWSGRKVFAEYASFRLEPESEYYKLRLGRYHGNAGDSFTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVVMMIRPNPNTFH
Expression Range	23-493aa
Protein Length	Full Length of Mature Protein
Mol. Weight	70.8kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Induces sprouting in endothelial cells through an autocrine and paracrine action.
Subcellular Location	Secreted.
Database References	HGNC: 490 OMIM: 605001 KEGG: hsa:23452 STRING: 9606.ENSP00000362524 UniGene: Hs.653262
Tissue Specificity	Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.

Gene Functions References

examination of the potential sources of circulating ANGPTL2 and its common pathological properties associated with various chronic inflammatory diseases (review). PMID: 29138671
Results showed that ANGPTL2 was highly expressed in glioma tissues and cell lines. Knockdown of ANGPTL2 reduced the proliferative and invasive abilities of glioma cells. PMID: 28247845
REVIEW: ANGPTL2 autocrine/paracrine signaling is a new factor in accelerating heart disease development in the aging. Here, we focus on current topics relevant to ANGPTL2 function in heart disease. PMID: 28867689
ANGPTL2 is a biomarker for liver fibrosis in chronic hepatitis B patients with normal to minimally raised ALT. PMID: 28962551
ANGPTL2 was positively associated with aortic stiffness after kidney transplantation. PMID: 28158589
Serum ANGPTL2 levels were significantly increased in non-small cell lung carcinoma patients, which could serve as a novel potential diagnostic and prognostic biomarker for NSCLC. PMID: 28164488
Results reveal a novel cascade comprising sequential P. gingivalis lipopolysaccharide --> ANGPTL2 --> integrin alpha5beta1 --> inflammatory cytokine induction, which might be responsible for inducing potent periodontal disorganization activity in gingival epithelial cells. Via this pathway, ANGPTL2 functions in the pathogenesis of periodontitis and contributes to prolonging chronic inflammation in patients with systemi... PMID: 28934245
In patients with type 2 diabetes, serum ANGPTL2 concentrations were independently associated with death and MACE. PMID: 27491833
Elevated serum ANGPTL2 levels are a novel risk factor for the development of CVD in the general population. This association is partially mediated by metabolic disorders and inflammation. PMID: 27365403
Increased ANGPTL2 expression contributes to proliferation and invasion of gastric cancer cells. PMID: 28058016
Aberrant expression of ANGPTL2 in cumulus cells is potentially associated with impaired oocyte developmental competence in polycystic ovary syndrome. PMID: 26829602
Reduced leukocyte DNA methylation in the promoter region of ANGPTL2 is associated with the pro-inflammatory environment that characterizes patients with post-ACS differently from age-matched healthy controls. Methylation of different CpGs in ANGPTL2 gene may prove to be a reliable biomarker of coronary disease PMID: 27101308
At 11-13 weeks in pregnancies that develop GDM, the serum concentration of ANGPTL2 is increased, and it can be combined with maternal factors to provide effective early screening for GDM. PMID: 27647189
ANGPTL2 and TGF-beta1 positively regulate each other as renal fibrosis progresses. PMID: 26806834
ANGPTL2 may be a useful marker for detecting early postoperative recurrence in patients with gastric cancer. PMID: 26254352
In lumbar spinal stenosis, Angptl2 promotes inflammation in ligamentum flavum(LF) tissue by activating IL-6 expression, leading to LF degeneration and hypertrophy. PMID: 25735609
ANGPTL2 promotes adipose tissue macrophage and T lymphocyte accumulation and leads to insulin resistance. PMID: 26132105
Serum ANGPTL2 in GC patients was significantly higher than for healthy controls. PMID: 26420253
Angptl2 induces proinflammatory responses in peritoneal macrophages and monocytes. PMID: 26435501
Serum ANGPTL2 concentration was associated with carotid atherosclerosis in patients with type 2 diabetes. PMID: 25889082
these findings are the first to suggest a considerable role for Angptl2 in the pathogenesis of unstable coronary disease in a clinical context PMID: 25999029
These results suggested that ANGPTL2 was a potential biomarker for gastric cancer. PMID: 25484242
Serum ANGPTL2 improves preoperative detection of LN metastasis in CRC PMID: 25964566
Serum ANGPTL2 is a novel diagnostic and recurrence-predictive biomarker in patients with colorectal cancer. PMID: 25294915
ANGPTL2 may be important in the acquisition of androgen independency and tumor progression of prostate cancer in an autocrine and/or paracrine manner via the integrin alpha5beta1 receptor. PMID: 25370833
Serum ANGPTL2 levels in patients with metastatic breast cancer were significantly higher than those in healthy subjects or in patients with ductal carcinoma in situ or non-metastatic invasive ductal carcinoma. PMID: 24585434
abnormal upregulation of ANGPTL2 in colorectal cancer is associated with miR-25 downregulation PMID: 25174582
This review aims at presenting an updated description of both the beneficial and deleterious biological properties of angptl2, in addition to its molecular signalling pathways and transcriptional regulation PMID: 25417860
Results show that ANGPTL2 antagonizes apoptosis by increasing Syk expression in colorectal cancer cells resistant to chemotherapy. PMID: 25287946
ANGPTL2 positively regulates endothelial colony forming cell vascular lumen formation. PMID: 24563071
findings demonstrate that preventing ANGPTL2 signaling stimulated by the tumor microenvironment could inhibit tumor cell migration and metastasis PMID: 24448647
expression of Angptl2 induced by mechanical stress in ligamentus flavum (LF) fibroblasts promotes LF tissue degeneration. PMID: 24465594
Endothelial cell-derived Angptl2 accelerates vascular inflammation by activating proinflammatory signaling in endothelial cells and increasing macrophage infiltration, leading to endothelial dysfunction and atherosclerosis progression. PMID: 24526691
Elevated serum Angptl2 is associated with the likelihood of CKD in the general population. PMID: 23739531
Angptl2 levels are elevated in patients with type 2 diabetes with an independent association between increasing Angptl2 levels and increasing levels of albuminuria. PMID: 23602322
periodic expression of ANGPTL2 is regulated by a molecular clock PMID: 23469106
In epicardial adipose tissue from coronary heart disease patients, ANGPTL2 expression was positively correlated with that of TNF-alpha. PMID: 23333801
Elevated serum ANGPTL2 levels were positively associated with the development of T2DM in a general population, independent of other risk factors including hs-CRP levels. PMID: 22966088
ANGPTL-2 and -3 have enhancing effect on human hematopoietic progenitor cell survival, effects requiring the CC domain of the ANGPTL molecules. PMID: 21983347
Macrophage-derived Angptl2 contributes to abdominal aortic aneurysm development by inducing inflammation and degradation of extracellular matrix in the vessel wall. PMID: 22556334
tumor cell-derived ANGPTL2 drives metastasis and provided an initial proof of concept for blockade of its action as a strategy to antagonize the metastatic process. PMID: 22345152
keratinocyte-derived Angptl2 functions in dermatomyositis pathogenesis by inducing chronic inflammation in skin tissue. PMID: 22281496
Angptl2 acts as an important rheumatoid synovium-derived inflammatory mediator in RA pathogenesis PMID: 20304962
The upregulation of ANGPTL2 in diabetic glomerulopathy shows a close relationship to abnormal microvasculature and endothelial inflammation. PMID: 17347581
epigenetic silencing by hypermethylation of the ANGPTL2 promoter leads to a loss of ANGPTL2 function, which may be a factor in the carcinogenesis of ovarian cancer in a stage-dependent manner. PMID: 18593905
Angiopoietin-like protein 2 (Angptl2) was secreted by adipose tissue and that its circulating level was closely related to adiposity, systemic insulin resistance, and inflammation in humans. PMID: 19723494

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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