Recombinant Human ANGPTL2 Protein

Name: Recombinant Human ANGPTL2 Protein
Brand: Beta LifeScience
SKU: BL-0331PS
Availability: InStock

Collections: Cytokines and growth factors, Other cytokines, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag	His
Host Species	Human
Synonym	Angiopoietin-related protein 2, Angiopoietin-like protein 2, ANGPTL2, ARP2, HARP.
Background	Angiopoietins belong to the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietins-1, 2 and 4 partake in the formation of blood vessels. ANGPTL2 displays angiogenic effects. Angiopoietin-like Protein 2 (ANGPTL2) is an anti-diabetic factor. ANGPTL2 induces sprouting in endothelial cells through an autocrine and paracrine action. ANGPTL2 increases insulin sensitivity in adipocytes. In addition, ANGPTL2 is a mediator of chronic adipose tissue inflammation. ANGPTL2 is widely expressed in the heart, small intestine, spleen and stomach. ANGPTL2 is also found in lower levels in the colon, ovary, adrenal gland, skeletal muscle and in prostate.
Description	ANGPTL2 Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain containing 481a.a. including a 10 a.a N-terminal His tag. The total molecular weight is 56kDa (calculated).
Source	E.coli
AA Sequence	MKHHHHHHASQEDGFEGTEE GSPREFIYLN RYKRAGESQD KCTYTFIVPQ QRVTGAICVN SKEPEVLLEN RVHKQELELL NNELLKQKRQ IETLQQLVEV DGGIVSEVKL LRKESRNMNS RVTQLYMQLL HEIIRKRDNA LELSQLENRI LNQTADMLQL ASKYKDLEHK YQHLATLAHN QSEIIAQLEE HCQRVPSARP VPQPPPAAPP RVYQPPTYNR IINQISTNEI QSDQNLKVLP PPLPTMPTLT SLPSSTDKPS GPWRDCLQAL EDGHDTSSIY LVKPENTNRL MQVWCDQRHD PGGWTVIQRR LDGSVNFFRN WETYKQGFGN IDGEYWLGLE NIYWLTNQGN YKLLVTMEDW SGRKVFAEYA SFRLEPESEY YKLRLGRYHG NAGDSFTWHN GKQFTTLDRD HDVYTGNCAH YQKGGWWYNA CAHSNLNGVW YRGGHYRSRY QDGVYWAEFR GGSYSLKKVV MMIRPNPNTF H.
Purity	>95.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	ANGPTL2 was filtered (0.4 µm) and lyophilized from 0.5mg/ml in 0.03M Acetate buffer pH 4.0.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.