Recombinant Human CD13 Protein (603 Met/Ile, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0656
Recombinant Human CD13 Protein (603 Met/Ile, His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0656
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001141.2 |
Synonym | APN, CD13, GP150, LAP1, P150, PEPN |
Background | Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas. |
Description | A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967,603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Lys 69 |
AA Sequence | Lys 69-Lys 967, 603 Met/Ile |
Molecular Weight | The recombinant human ANPEP protein consists of 910 a.a. and has a predicted molecular mass of 104.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhANPEP is approximately 135-140 kDa due to glycosylation. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2,500 pmoles/min/ug. |
Formulation | Lyophilized from sterile PBS, pH 7.3. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |